Lentiviral vector transduction provides nonspecific immunogenicity for syngeneic tumor models

Autor: Avishek Bhuniya, Dhamotharan Pattarayan, Da Yang
Rok vydání: 2022
Předmět:
Zdroj: Mol Carcinog
ISSN: 1098-2744
0899-1987
Popis: Lentivirus-based transduction systems are widely used in biological science and cancer biology, including cancer immunotherapy. However, in in vivo transplanted tumor model, the immunogenicity of these transduced cells was not appropriately addressed. Here we used empty vector transduced mouse melanoma (B16) and carcinoma (Lewis lung Carcinoma) cells transplanted tumor model to study the immune response due to the transduction processes. We showed that the overall in vivo tumor growth rate gets reduced in transduced cells only in immune-competent mice but not in nude mice. This data indicates the involvement of the immune system in the in vivo tumor growth restriction in the transduced group. Further studies showed that specific activation of CD8(+) T cells might be responsible for restricted tumor growth. Mechanistically, transduced tumor cells show the higher activity of type I interferon, which might play an essential role in this activation. Overall, our data indicates the modulation of the immune system by lentiviral vector transduced tumor cells, which required further studies to explore the mechanisms and better understand the biological significance. Our data also indicates the importance of considering the immunogenicity of transduced cells when analyzing in vivo results, especially in studies related to immunotherapy.
Databáze: OpenAIRE
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