Insensitivity of well-conditioned mature sheep to central administration of a leptin receptor antagonist
| Autor: | Stanley M. Hileman, Arieh Gertler, RA Ehrhardt, Yves R. Boisclair, Andreas Foskolos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Blood Glucose
medicine.medical_specialty sheep medicine.medical_treatment brain adaptive metabolism Fatty Acids Nonesterified Biology leptin SF1-1100 Random Allocation Insulin resistance Internal medicine medicine Animals Insulin Leptin receptor Triiodothyronine Leptin Insulin tolerance test digestive oral and skin physiology Antagonist medicine.disease Animal Feed Animal culture Infusions Intraventricular Endocrinology Receptors Leptin Female Animal Science and Zoology leptin receptor antagonist hormones hormone substitutes and hormone antagonists Signal Transduction Hormone |
| Zdroj: | Animal, Vol 9, Iss 11, Pp 1852-1858 (2015) |
| ISSN: | 1751-7311 |
| Popis: | Ruminants remain productive during the energy insufficiency of late pregnancy or early lactation by evoking metabolic adaptations sparing available energy and nutrients (e.g. higher metabolic efficiency and induction of insulin resistance). A deficit in central leptin signaling triggers these adaptations in rodents but whether it does in ruminants remains unclear. To address this issue, five mature ewes were implanted with intracerebroventricular (ICV) cannula in the third ventricle. They were used in two experiments with an ovine leptin antagonist (OLA) when well-conditioned (average body condition score of 3.7 on a 5 point scale). The first experiment tested the ability of OLA to antagonize leptin under in vivo conditions. Ewes received continuous ICV infusion of artificial cerebrospinal fluid (aCSF), ovine leptin (4 µg/h) or the combination of ovine leptin (4 µg/h) and its mutant version OLA (40 µg/h) for 48 h. Dry matter intake (DMI) was measured every day and blood samples were collected on the last day of infusion. ICV infusion of leptin reduced DMI by 24% (P |
| Databáze: | OpenAIRE |
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