Plasma microRNAs as potential biomarkers for non-small-cell lung cancer
| Autor: | Xing Lingxiao, Jipei Liao, Maria A. Guarnera, Jun Shen, Zhengran Jiang, Changwan Larry Lu, Ruth L. Katz, Feng Jiang, Yuping Mei, Nevins W. Todd, Lei Yu, Amy Chou, Hong-Bin Fang, Howard Zhang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Oncology medicine.medical_specialty Lung Neoplasms Microarray diagnosis Adenocarcinoma of Lung Adenocarcinoma Biology Sensitivity and Specificity Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Internal medicine microRNA Biomarkers Tumor medicine Carcinoma Humans Stage (cooking) Lung cancer Molecular Biology plasma Aged Neoplasm Staging 030304 developmental biology 0303 health sciences Lung Reverse Transcriptase Polymerase Chain Reaction Reproducibility of Results Cell Biology Middle Aged medicine.disease respiratory tract diseases 3. Good health lung cancer MicroRNAs Logistic Models medicine.anatomical_structure Real-time polymerase chain reaction 030220 oncology & carcinogenesis Carcinoma Squamous Cell qRT-PCR Female Research Article |
| Zdroj: | Laboratory Investigation; a Journal of Technical Methods and Pathology |
| ISSN: | 0023-6837 |
| Popis: | Non-small-cell lung cancer (NSCLC) is the leading cause of cancer-related death. Developing minimally invasive techniques that can diagnose NSCLC, particularly at an early stage, may improve its outcome. Using microarray platforms, we previously identified 12 microRNAs (miRNAs) the aberrant expressions of which in primary lung tumors are associated with early-stage NSCLC. Here, we extend our previous research by investigating whether the miRNAs could be used as potential plasma biomarkers for NSCLC. We initially validated expressions of the miRNAs in paired lung tumor tissues and plasma specimens from 28 stage I NSCLC patients by real-time quantitative reverse transcription PCR, and then evaluated diagnostic value of the plasma miRNAs in a cohort of 58 NSCLC patients and 29 healthy individuals. The altered miRNA expressions were reproducibly confirmed in the tumor tissues. The miRNAs were stably present and reliably measurable in plasma. Of the 12 miRNAs, five displayed significant concordance of the expression levels in plasma and the corresponding tumor tissues (all r>0.850, all P |
| Databáze: | OpenAIRE |
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