Downregulation of lncRNA ZFAS1 and upregulation of microRNA-129 repress endocrine disturbance, increase proliferation and inhibit apoptosis of ovarian granulosa cells in polycystic ovarian syndrome by downregulating HMGB1
| Autor: | Zhi-fen Zhang, Yue-qun Chen, Hong-li Zhu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
endocrine system endocrine system diseases Down-Regulation Apoptosis chemical and pharmacologic phenomena HMGB1 01 natural sciences 03 medical and health sciences Downregulation and upregulation microRNA Genetics Humans Secretion HMGB1 Protein Cell Proliferation 030304 developmental biology Zinc finger 0303 health sciences Granulosa Cells biology Transfection Polycystic ovary female genital diseases and pregnancy complications Up-Regulation MicroRNAs Disease Progression biology.protein Cancer research Female RNA Long Noncoding Polycystic Ovary Syndrome 010606 plant biology & botany |
| Zdroj: | Genomics. 112:3597-3608 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2020.04.011 |
| Popis: | Objective The objective was to find the role of long-non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1)/microRNA (miR)-129/high-mobility group box protein 1 (HMGB1) axis in polycystic ovary syndrome (PCOS). Methods Ovarian granulosa cells from non-PCOS patients and PCOS patients were collected, and HMGB1, miR-129 and lncRNA ZFAS1 expression were detected. Ovarian granulosa cells were transfected with si-ZFAS1 or miR-129 mimics to verify their roles in P4 and E2 secretion, and the biological functions of ovarian granulosa cells. Results LncRNA ZFAS1 and HMGB1 were elevated, while miR-129 was down-regulated in ovarian granulosa cells of PCOS patients. Down-regulated lncRNA ZFAS1 or overexpressed miR-129 could decrease HMGB1 expression, increase P4 and E2 secretion, promote proliferation activity while inhibit apoptosis of ovarian granulosa cells in PCOS. Conclusion LncRNA ZFAS1 could bind to miR-129 to promote HMGB1 expression, thereby affecting the endocrine disturbance, proliferation and apoptosis of ovarian granulosa cells in PCOS. |
| Databáze: | OpenAIRE |
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