Cis- andtrans-acting factors regulating transcription of the BGT1 gene in response to hypertonicity
| Autor: | Ching-Pu Chen, Seung Kyoon Woo, Joseph S. Handler, Hiroshi Miyakawa, Stephen C. Dahl, H. Moo Kwon |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
GABA Plasma Membrane Transport Proteins
Transcription Genetic Physiology Hypertonic Solutions Molecular Sequence Data Response element E-box Biology Kidney Cell Line Dogs Sp3 transcription factor Animals Humans Enhancer Base Sequence General transcription factor Stereoisomerism Promoter TCF4 Molecular biology Enhancer Elements Genetic Mutation TAF2 Carrier Proteins Transcription Factors |
| Zdroj: | American Journal of Physiology-Renal Physiology. 274:F753-F761 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.1998.274.4.f753 |
| Popis: | We have previously identified a tonicity-responsive enhancer (TonE) in the promoter region of the canine BGT1 gene. TonE mediates hypertonicity-induced stimulation of transcription. Here, we characterize TonE and TonE binding proteins (TonEBPs) to provide a biochemical basis for cloning of the TonEBPs. Mutational analysis applied to both hypertonicity-induced stimulation of transcription and TonEBP binding reveals that TonE is 11 base pairs in length, with the consensus sequence of (C/T)GGAAnnn(C/T)n(C/T). Activity of the TonEBPs increases in response to hypertonicity with a time course similar to that of transcription of the BGT1 gene. Studies with inhibitors indicate that translation, but not transcription, is required for activation of the TonEBPs. Phosphorylation is required for the stimulation of transcription but not for activation of DNA binding by the TonEBPs. In vivo methylation by dimethyl sulfate reveals that the TonE site of the BGT1 gene is protected with a time course like that of activity of the TonEBPs and activation of transcription. Ultraviolet cross-linking indicates that the TonEBPs share a DNA binding subunit of 200 kDa. |
| Databáze: | OpenAIRE |
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