Neurotrophin-3, but not nerve growth factor, promotes survival of human intestinal mast cells
| Autor: | John Bienenstock, Stephan C. Bischoff, Thomas Gebhardt, H. Worthmann, Axel Lorentz, J. Hoppe, U. Hesse |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Cell Survival Physiology Stem cell factor Neurotrophin-3 Biology Tropomyosin receptor kinase C chemistry.chemical_compound Neurotrophin 3 Internal medicine Nerve Growth Factor medicine Humans Mast Cells Receptor trkA Leukotriene C4 Reverse Transcriptase Polymerase Chain Reaction Endocrine and Autonomic Systems Gastroenterology Glyceraldehyde-3-Phosphate Dehydrogenases Mast cell Immunohistochemistry Cell biology Intestines Endocrinology medicine.anatomical_structure Nerve growth factor nervous system chemistry biology.protein RNA Histamine Neurotrophin |
| Zdroj: | Neurogastroenterology & Motility. 19:301-308 |
| ISSN: | 1365-2982 1350-1925 |
| DOI: | 10.1111/j.1365-2982.2007.00899.x |
| Popis: | Neurotrophins are potent regulators of neuronal cell survival and function. Nerve growth factor (NGF) was shown to reduce apoptosis in cord blood-derived mast cells. Here, we examined the effect of the neurotrophins NGF and neurotrophin (NT)-3 on survival and mediator release of human intestinal mast cells. Mast cells isolated from normal intestinal tissue were cultured in the presence of NGF, NT-3, or stem cell factor (SCF) alone or in the presence of SCF together with each neurotrophin. NGF or NT-3 alone did not promote mast cell survival. In contrast, mast cell recovery was increased twofold when mast cells were cultured with NT-3 in addition to SCF for 14 days compared with control. Mast cell recovery was further increased following a combined addition of NT-3, SCF and IL-4. NT-3 mediated mast cell growth was dependent on the primary receptor for NT-3 TrkC. NGF in combination with SCF or with SCF and IL-4 showed no effect on mast cell survival. Histamine release and histamine content per mast cell remained unchanged, whereas leukotriene C4 release decreased if mast cells were cultured with NGF or NT-3 in addition to SCF. In summary, NT-3 affects mature human mast cells by promoting mast cell survival, whereas NGF does not. |
| Databáze: | OpenAIRE |
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