Differential expression of growth factors at the cellular level in virus-infected brain
| Autor: | Anna Zborek, Mikhail Prosniak, Hilary Koprowski, Timothy W. Phares, Gwen S. Scott, Anirban Roy, D. Craig Hooper |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Gene isoform
Mice Inbred C3H Multidisciplinary Base Sequence Rabies virus Brain Fluorescent Antibody Technique Biological Sciences Biology Fibroblast growth factor medicine.disease_cause Virology Virus Mice Viral replication Gene expression medicine Animals RNA Messenger Growth Substances Gene DNA Primers Laser capture microdissection |
| Zdroj: | Proceedings of the National Academy of Sciences. 100:6765-6770 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0430999100 |
| Popis: | The contribution of host factors to rabies virus (RV) transcription/replication and axonal/transsynaptic spread is largely unknown. We previously identified several host genes that are up-regulated in the mouse brain during RV infection, including neuroleukin, which is involved in neuronal growth and survival, cell motility, and differentiation, and fibroblast growth factor homologous factor 4 (FHF4), which has been implicated in limb and nervous system development. In this study, we used real-time quantitative RT-PCR to assess the expression of mRNAs specific for neuroleukin, the two isoforms of FHF4 (FHF4-1a and -1b) encoded by the FHF4 gene, and N protein of RV in neurons and astrocytes isolated by laser capture microdissection from mouse brains infected with the laboratory-adapted RV strain CVS-N2c or with a street RV of silver-haired bat origin. Differences in the gene expression patterns suggest that the capacity of RV strains to infect nonneuronal cells and differentially modulate host gene expression may be important in virus replication and spread in the CNS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|