Cross-platform mechanical characterization of lung tissue
| Autor: | D. Ezra Aurian-Blajeni, Shelly R. Peyton, Nathan P. Birch, Alfred J. Crosby, Carey E. Dougan, Jessica D. Schiffman, Aritra Nath Kundu, Samuel R. Polio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Respiratory System Sus scrofa lcsh:Medicine Modulus 02 engineering and technology Stiffness Freezing Medicine and Health Sciences Biomechanics lcsh:Science Materials Lung Lung Compliance Microscale chemistry 0303 health sciences Microscopy Multidisciplinary Physics Classical Mechanics 021001 nanoscience & nanotechnology Atomic Force Microscopy Biomechanical Phenomena Connective Tissue Cavitation Physical Sciences Female medicine.symptom Anatomy 0210 nano-technology Rheology Research Article Materials science Tissue Mechanics Amorphous Solids Materials Science Material Properties Uniaxial tension Biophysics In Vitro Techniques Research and Analysis Methods Continuum Mechanics Models Biological 03 medical and health sciences Elastic Modulus medicine Mechanical Properties Animals Humans Elasticity (economics) Elastic modulus Bronchioles 030304 developmental biology Scanning Probe Microscopy lcsh:R Biology and Life Sciences Small amplitude 030104 developmental biology Biological Tissue Cartilage Mixtures Respiratory Mechanics lcsh:Q Lung tissue Gels Biomedical engineering |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 10, p e0204765 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Published data on the mechanical strength and elasticity of lung tissue is widely variable, primarily due to differences in how testing was conducted across individual studies. This makes it extremely difficult to find a benchmark modulus of lung tissue when designing synthetic extracellular matrices (ECMs). To address this issue, we tested tissues from various areas of the lung using multiple characterization techniques, including micro-indentation, small amplitude oscillatory shear (SAOS), uniaxial tension, and cavitation rheology. We report the sample preparation required and data obtainable across these unique but complimentary methods to quantify the modulus of lung tissue. We highlight cavitation rheology as a new method, which can measure the modulus of intact tissue with precise spatial control, and reports a modulus on the length scale of typical tissue heterogeneities. Shear rheology, uniaxial, and indentation testing require heavy sample manipulation and destruction; however, cavitation rheology can be performed in situ across nearly all areas of the lung with minimal preparation. The Young’s modulus of bulk lung tissue using microindentation (1.9±0.5 kPa), SAOS (3.2±0.6 kPa), uniaxial testing (3.4±0.4 kPa), and cavitation rheology (6.1±1.6 kPa) were within the same order of magnitude, with higher values consistently reported from cavitation, likely due to our ability to keep the tissue intact. Although cavitation rheology does not capture the non-linear strains revealed by uniaxial testing and SAOS, it provides an opportunity to measure mechanical characteristics of lung tissue on a microscale level on intact tissues. Overall, our study demonstrates that each technique has independent benefits, and each technique revealed unique mechanical features of lung tissue that can contribute to a deeper understanding of lung tissue mechanics. |
| Databáze: | OpenAIRE |
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