Turn-on, fluorescent nuclear stains with live cell compatibility
| Autor: | Jens Wigenius, James N. Wilson, Demar R. G. Pitter, Fredrik Westerlund, Adrienne S. Brown, James D. Baker |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Indoles
Staining and Labeling Chemistry Lasers Organic Chemistry Intercalation (chemistry) Analytical chemistry Molecular Conformation Electronic structure DNA Laser Linear dichroism Biochemistry Fluorescence law.invention chemistry.chemical_compound Membrane Spectrometry Fluorescence Models Chemical law Benzimidazoles DAPI Physical and Theoretical Chemistry Spectroscopy Fluorescent Dyes |
| Zdroj: | Organic letters. 15(6) |
| ISSN: | 1523-7052 |
| Popis: | DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|