Semi-continuous production of 2-keto-gluconic acid by Pseudomonas fluorescens AR4 from rice starch hydrolysate
| Autor: | Shi-Lian Yu, Yan-Zheng Zhou, Qiang Zhou, Lei Sun, Wenjing Sun, Fengjie Cui |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Environmental Engineering
biology Renewable Energy Sustainability and the Environment Starch Hydrolysis Oryza Bioengineering Pseudomonas fluorescens General Medicine biology.organism_classification Gluconates Hydrolysate Culture Media chemistry.chemical_compound chemistry Biochemistry Yield (chemistry) Fermentation Gluconic acid Food science Waste Management and Disposal Bacteria |
| Zdroj: | Bioresource Technology. 110:546-551 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2012.01.040 |
| Popis: | 2-Keto-gluconic acid (2KGA) was produced in a semi-continuous process using Pseudomonas fluorescens AR4 and rice starch hydrolysate (RSH). The bacterium was cultured in medium with an initial glucose concentration of 170g/L supplied as RSH. Once the glucose level had dropped to 20g/L, 60% of the culture volume was replaced with fresh medium containing 190g/L of glucose in the form of RSH. After an additional two cycles of growth and media replacement, a total of 476.88g/L of glucose was consumed and 444.96g/L of 2KGA was produced. A total productivity of 6.74g/L and a yield of 0.93g/g were obtained. These findings suggest that P. fluorescens AR4 is suitable for the production of commercially acceptable levels of 2KGA in semi-continuous culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect