Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
| Autor: | O. G. Pinaeva, N. A. Tsimbalist, E N Sazonova, Yu. B. Malofey, P. K. Tarasov, D. A. Kupriyanova, O. A. Lebed’ko |
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| Rok vydání: | 2021 |
| Předmět: |
Arginine
Peptide medicine.disease_cause Nitric Oxide Protective Agents General Biochemistry Genetics and Molecular Biology peptide analogue of leu-enkephalin Nitric oxide chemistry.chemical_compound medicine Animals Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) Rats Wistar Lung Cells Cultured chemistry.chemical_classification Chemistry Superoxide General Medicine Hydrogen Peroxide Fibroblasts Leu-enkephalin Molecular biology In vitro Amino acid Rats Oxidative Stress NG-Nitroarginine Methyl Ester cytoprotective effect Cytoprotection Oxidative stress Enkephalin Leucine |
| Zdroj: | Bulletin of Experimental Biology and Medicine |
| ISSN: | 1573-8221 |
| Popis: | Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53+ cells (by 40.2%) induced by exposure to H2O2 (60 μM). The cytoprotective effect of NALE was potentiated by NO synthase inhibitor L-NAME (1 mM): the number of p53+ cells decreased by 65.3% and morphometric parameters of the cell nuclei and nucleoli were improved. Incubation of pulmonary fibroblasts culture with peptide G (Phe-D-Ala-Gly-Phe-Leu-Gly, 0.1 μM) also significantly reduced the damaging effect of H2O2: the number of p53+ cells decreased by 73.5%, the area of cell nuclei returned to normal, and generation of superoxide anion-radical decreased by 18.4%. These results indicate that C-terminal amino acid Arg and activation of NO synthase are not involved in the direct cytoprotective effect of NALE. |
| Databáze: | OpenAIRE |
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