Immune modulation by chronic exposure to waterpipe smoke and immediate-early gene regulation in murine lungs
| Autor: | Marielle C. Brinkman, Wayne Mitzner, Shyam Biswal, Hermes Reyes-Caballero, Juhyung Woo, Jeffrey Loube, Youngshim Choi, Justin P. Edwards, Vinesh Vinayachandran, Thomas Sussan, Ian Sanchez, Bongsoo Park |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chemokine
Nicotine Health (social science) Water Pipe Smoking Smoking Water Pipes Pulmonary function testing Transcriptome 03 medical and health sciences Mice 0302 clinical medicine Immune system medicine Animals 030212 general & internal medicine Genes Immediate-Early Lung 030304 developmental biology Smoke 0303 health sciences biology business.industry Public Health Environmental and Occupational Health Mice Inbred C57BL medicine.anatomical_structure Immunology biology.protein Female business Immediate early gene Biomarkers medicine.drug |
| Zdroj: | Tobacco control. 29(Suppl 2) |
| ISSN: | 1468-3318 |
| Popis: | ObjectiveWe investigated the effects of chronic waterpipe (WP) smoke on pulmonary function and immune response in a murine model using a research-grade WP and the effects of acute exposure on the regulation of immediate-early genes (IEGs).MethodsWP smoke was generated using three WP smoke puffing regimens based on the Beirut regimen. WP smoke samples generated under these puffing regimens were quantified for nicotine concentration. Mice were chronically exposed for 6 months followed by assessment of pulmonary function and airway inflammation. Transcriptomic analysis using RNAseq was conducted after acute exposure to characterise the IEG response. These biomarkers were then compared with those generated after exposure to dry smoke (without water added to the WP bowl).ResultsWe determined that nicotine composition in WP smoke ranged from 0.4 to 2.5 mg per puffing session. The lung immune response was sensitive to the incremental severity of chronic exposure, with modest decreases in airway inflammatory cells and chemokine levels compared with air-exposed controls. Pulmonary function was unmodified by chronic WP exposure. Acute WP exposure was found to activate the immune response and identified known and novel IEG as potential biomarkers of WP exposure.ConclusionChronic exposure to WP smoke leads to immune suppression without significant changes to pulmonary function. Transcriptomic analysis of the lung after acute exposure to WP smoke showed activation of the immune response and revealed IEGs that are common to WP and dry smoke, as well as pools of IEGs unique to each exposure, identifying potential biomarkers specific to WP exposure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|