Effects of lysed Enterococcus faecalis FK-23 on allergen-induced serum antibody responses and active cutaneous anaphylaxis in mice
| Autor: | Keisuke Enomoto, Taro Shirakawa, T. Shimada, Hiroshi Yasueda, Tadao Enomoto, Chie Motonaga, Lei Cheng, Akiko Yamasaki, M. Ide |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Allergy
Cryptomeria Immunology Administration Oral Spleen medicine.disease_cause Immunoglobulin E Enterococcus faecalis Microbiology Mice Allergen Immune system medicine Animals Immunology and Allergy Anaphylaxis Cells Cultured Skin Tests Antigens Bacterial Mice Inbred BALB C biology Allergens medicine.disease biology.organism_classification medicine.anatomical_structure Immunoglobulin G biology.protein Cytokines Pollen Female Muramidase Antibody |
| Zdroj: | Clinical Experimental Allergy. 34:1784-1788 |
| ISSN: | 1365-2222 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2004.02092.x |
| Popis: | Summary Background Our previous studies have presented evidence that lysed Enterococcus faecalis FK-23(LFK), a lysozyme and heat-treated probiotic product, can inhibit allergen-induced local accu-mulation of eosinophils in mice.Objective The purpose of this experimental study was to evaluate the influence of orallyadministrated LFK on the host immune responses.Methods BALB/c mice were sensitized subcutaneously, and challenged intraperitoneally by cedarpollen allergen. Blood and spleen samples were collected after oral administration of LFK 60mg/dayfor 21 days. The serum levels of total and allergen-specific IgE and IgG2a antibodies and theproduction of IL-4, IL-5 and IFN-g generated by allergen-stimulated cultured splenocytes weredetermined. Additionally, the effect of LFK on active cutaneous anaphylaxis (ACA) induced byovalbumin (OVA) challenge in mice was measured after 28 days LFK treatment.Results No significant differences in serum immunoglobulin levels, as well as in cytokineproduction of splenocytes were observed between LFK-treated and control mice (P40.05). Therewas, however, anincreasingtendency of allergen-specific IgG2a levelin mice afterLFKtreatmentfor21 days compared with controls (P50.060). Furthermore, the serum ratio of specific IgE to IgG2awas found to be significantly decreased in the LFK group (P50.005). In addition, a significantinhibition of OVA-induced ACA reaction was observed in mice that had been fed for 28 days withLFK compared with control mice (P50.008).Conclusion These results suggest that LFK shows an anti-inflammatory effect, which may be partof the mechanism for protection against IgE-mediated allergy.Keywords allergy, cutaneous anaphylaxis, cytokines, Enterococcus faecalis, immunoglobulins, lacticacid bacteriaSubmitted 3 September 2003; revised 1 March 2004; accepted 20 June 2004 |
| Databáze: | OpenAIRE |
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