Influence of Shear on Protein Crystallization under Constant Shear Conditions
| Autor: | Sudha Chinnu, Marzena Krzek, Dominique Maes, Pierre Philippe Gelin, Sander Stroobants, Wim De Malsche, Manly Callewaert, James F. Lutsko, Iwona Ziemecka |
|---|---|
| Přispěvatelé: | Structural Biology Brussels, Department of Bio-engineering Sciences, Faculty of Sciences and Bioengineering Sciences, Faculty of Engineering, Chemical Engineering and Industrial Chemistry, Centre for Molecular Separation Science & Technology, Chemical Engineering and Separation Science |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Physique de l'état condense [struct. électronique
etc.] Materials science Chemistry(all) 010405 organic chemistry Microfluidics Physique de l'état solide Biologie moléculaire General Chemistry Mechanics Biophysique 010402 general chemistry Condensed Matter Physics 01 natural sciences 0104 chemical sciences Physics::Fluid Dynamics Constant linear velocity Materials Science(all) Shear (geology) General Materials Science Lid driven Protein crystallization Shear flow |
| Zdroj: | Crystal growth & design, 20 (3 |
| Popis: | A novel microfluidic device that subjects a solution to a constant shear flow was developed. By taking advantage of the linear velocity profile in a lid driven flow configuration, small volumes (10-5 L) can be subjected to a constant shear profile with a shear rate between 0.1 and 100 s-1 at accurately controlled temperatures between 20 and 50 °C. The tunable shear can be maintained for extensive and fully controlled times. A dedicated microscope setup for visualization enables the on-chip detection of micron-sized crystals, particles, and aggregates. The influence of shear on the crystallization process of the reference protein lysozyme was studied. The results indicate that shear rates between 1 and 10 s-1 decrease solubility and promote nucleation not only in the supersaturated and metastable zones of the phase diagram, but also in the undersaturated zone. A monotonically increasing nucleation rate was observed for shear rates between 1 and 10 s-1. It is anticipated that the presented methodology can shed light on a variety of phase transitions that are influenced by flow. info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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