Tyk2 expression and its signaling enhances the invasiveness of prostate cancer cells
| Autor: | Takashi Nakagawa, Satoru Muto, Shigeo Horie, Hisamitsu Ide, Yutaka Kamiyama, Yuichi Terado |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Biophysics Biology Biochemistry Cell Line Metastasis Prostate cancer Cancer stem cell Interferon medicine Humans Neoplasm Invasiveness Molecular Biology Cell Proliferation TYK2 Kinase Kinase Prostatic Neoplasms Cell Biology medicine.disease Gene Expression Regulation Neoplastic Cancer cell Cancer research Signal transduction Tyrosine kinase Signal Transduction medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 369:292-296 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.08.160 |
| Popis: | Protein tyrosine kinase plays a central role in the proliferation and differentiation of various types of cells. One of these protein kinases, Tyk2, a member of the Jak family kinases, is known to play important roles in receptor signal transduction by interferons, interleukins, growth factors, and other hormones. In the present study, we investigated Tyk2 expression and its role in the growth and invasiveness of human prostate cancer cells. We used a small interfering RNA targeting Tyk2 and an inhibitor of Tyk2, tyrphostin A1, to suppress the expression and signaling of Tyk2 in prostate cancer cells. We detected mRNAs for Jak family kinases in prostate cancer cell lines by RT-PCR and Tyk2 protein in human prostate cancer specimens by immunohistochemistry. Inhibition of Tyk2 signaling resulted in attenuation of the urokinase-type plasminogen activator-enhanced invasiveness of prostate cancer cells in vitro without affecting the cellular growth rate. These results suggest that Tyk2 signaling in prostate cancer cells facilitate invasion of these cells, and interference with this signaling may be a potential therapeutic pathway. |
| Databáze: | OpenAIRE |
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