LncRNA NEAT1 regulates MMP-16 by targeting miR-200a/b to aggravate inflammation in asthma
| Autor: | Niu Ding, Xiao-Jun Duan, Ji-Yan Zhang, Yan-Ping Chen, Xi Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Inflammation
medicine.diagnostic_test Chemistry Cell growth Immunology Cell Interleukin Apoptosis Matrix Metalloproteinase 16 Asthma Flow cytometry MicroRNAs medicine.anatomical_structure Cancer research medicine Immunology and Allergy Gene silencing Humans Tumor necrosis factor alpha RNA Long Noncoding medicine.symptom Cell Proliferation |
| Zdroj: | Autoimmunity. 54(7) |
| ISSN: | 1607-842X |
| Popis: | Asthma is a common respiratory disease which is characterized by persistent airway inflammation. Abnormal expression of long non-coding RNAs (lncRNAs) is observed in asthma. However, whether lncRNA nuclear-enriched abundant transcript 1 (NEAT1) regulates asthmatic inflammation and its mechanism still needs to be further investigated. The expression levels of inflammatory factors (tumour necrosis factor (TNF)-α, interleukin (IL)-4, IL-13, and IL-10) were detected using reverse transcription quantitative real-time PCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). MTT and flow cytometry assays were employed to determine cell proliferation and apoptosis, respectively. Dual luciferase reporter assay was performed to verify the relationship between miR-200a/b and MMP-16 or NEAT1. NEAT1 silencing markedly reduced TNF-α, IL-4, and IL-13 levels, while elevated IL-10 expression, suppressed cell proliferation, and promoted cell apoptosis. However, NEAT1 overexpression elicited the opposite effects on cell proliferation and inflammation cytokines secretion. What is more, NEAT1 negatively regulated miR-200a/b expression, and MMP16 was a target gene of miR-200a/b. miR-200a/b overexpression suppressed inflammation, cell proliferation, and enhanced cell apoptosis through regulation of MMP16. Moreover, MMP-16 overexpression or miR-200a/b inhibition abolished the regulatory effect of sh-NEAT1 on cell inflammation and apoptosis in BEAS-2B cells. NEAT1 acted as the role of sponge for miR-200a/b to regulate MMP-16 expression, thereby promoting asthma progression, suggesting that NEAT1 might have great potential as therapeutic target for asthma. |
| Databáze: | OpenAIRE |
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