The microbial community of a biofilm lining the wall of a pristine cave in Western New Guinea
| Autor: | Marco Ventura, Marco Tescari, Paolo Turrini, Mattia Pirolo, Gabriele Andrea Lugli, Paolo Visca, Daniela Visaggio, Emanuela Frangipani |
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| Přispěvatelé: | Turrini, P., Tescari, M., Visaggio, D., Pirolo, M., Lugli, G. A., Ventura, M., Frangipani, E., Visca, P. |
| Rok vydání: | 2020 |
| Předmět: |
DNA
Bacterial MLSA Firmicutes Microbial Sensitivity Tests Microbiology Actinobacteria Antibiotics Culturomics Karst caves MLSA Shotgun sequencing 16S rRNA 03 medical and health sciences Cave Pseudomonas RNA Ribosomal 16S Drug Resistance Bacterial Proteobacteria Culturomic Extreme environment 16S rRNA Soil Microbiology 030304 developmental biology New Guinea 0303 health sciences geography geography.geographical_feature_category Bacteria biology 030306 microbiology Ecology Microbiota Antibiotic Sequence Analysis DNA biology.organism_classification Shotgun sequencing Acidobacteria Anti-Bacterial Agents Caves Microbial population biology Metagenomics Biofilms Metagenome Karst cave |
| Zdroj: | Microbiological Research. 241:126584 |
| ISSN: | 0944-5013 |
| DOI: | 10.1016/j.micres.2020.126584 |
| Popis: | Caves are extreme environments inhabited by microbial communities adapted to thrive oligotrophic conditions. Cave microbes are organised in complex ecological networks and have developed survival strategies involving the production and release of a large variety of secondary metabolites, including antibiotic-like compounds. In this study, the structure and the metabolic features of a biofilm-like microbial community lining the walls of a pristine karst cavity (the Yumugi river cave) located in a remote region of the Western New Guinea were investigated. 16S rRNA and shotgun sequence analyses highlighted the prevalence of chemoorganotrophic phyla (Proteobacteria, Actinobacteria, Firmicutes and Acidobacteria), consistent with metabolic predictions inferred from the cave metagenome analysis. Few clinically relevant antimicrobial resistance genes were detected. A culture-based approach allowed the isolation of some heterotrophic members of the bacterial community, and antimicrobial susceptibility testing revealed an overall high level of resistance to different antimicrobials classes. Isolates presumptively representing new uncharacterized members of genus Pseudomonas displayed interesting antibiotic properties against Gram-positive indicator strains. Our work supports the hypothesis that caves represent a reservoir for new bacterial species and drug discovery research. |
| Databáze: | OpenAIRE |
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