Is intracytoplasmic sperm (ICSI) better than traditional in vitro fertilization (IVF): confirmation of higher blastocyst rates per oocyte using a split insemination design

Autor: Shunping Wang, May-Tal Sauerbrun-Cutler, Warren J. Huber, Chloe Shen, Richard J. Hackett, Ruben Alvero, Phinnara Has
Rok vydání: 2020
Předmět:
Adult
Male
0301 basic medicine
Pregnancy Rate
medicine.medical_treatment
Oocyte Retrieval
Fertilization in Vitro
Insemination
Intracytoplasmic sperm injection
Andrology
03 medical and health sciences
0302 clinical medicine
Human fertilization
Pregnancy
Genetics
Humans
Medicine
Sperm Injections
Intracytoplasmic
Blastocyst
Assisted Reproduction Technologies
reproductive and urinary physiology
Genetics (clinical)
Retrospective Studies
030219 obstetrics & reproductive medicine
In vitro fertilisation
urogenital system
business.industry
Obstetrics and Gynecology
Retrospective cohort study
General Medicine
Embryo Transfer
Oocyte
Sperm
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
Research Design
embryonic structures
Female
business
therapeutics
Developmental Biology
Zdroj: J Assist Reprod Genet
ISSN: 1573-7330
1058-0468
Popis: PURPOSE: To explore the effects of traditional vs. intracytoplasmic sperm injection (ICSI) insemination method on the outcome of high-quality blastocyst development in a split sibling oocyte cohort. METHODS: In this retrospective cohort study, we analyzed 62 ICSI/IVF split cycles. Sibling oocytes were randomly assigned to ICSI or IVF insemination. Two hundred thirty-four ICSI-only cycles and 152 IVF-only cycles were also analyzed for comparison. Blastocysts were graded by Gardner’s embryo grading and were considered a high-quality blastocyst if 3BB or better (Gardner 1999). RESULTS: In the ICSI/IVF split group, (1) ICSI oocytes had a higher fertilization rate per oocyte allocated (73% vs 62%, p < 0.001), (2) more high-quality day 2 embryos (69% vs 55%, p < 0.005), (3) ICSI oocytes had a lower blastulation rate per 2PN (46% vs 54%, p < 0.05), but a higher blastulation rate when calculated per oocyte allocated (40% vs 32%, p < 0.05). The ICSI-only group had a lower fertilization rate (65% vs 70%, p < 0.001) but more high-quality day 2 embryos in comparison to the IVF-only group (68% vs 64%, p < .05). The total high-quality blastulation rate was higher for the IVF-only group per 2PN (49% vs 43%, p < 0.05) and per oocyte retrieved (34% vs 28%, p < 0.05). CONCLUSIONS: This distinctive IVF/ICSI sibling oocyte split design demonstrated a higher-quality blastulation rate in the IVF group compared to the ICSI group when calculated per 2PN, but not per oocyte allocated to each insemination procedure.
Databáze: OpenAIRE
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