Magnetically Agitated Nanoparticle-Based Batch Reactors for Biocatalysis with Immobilized Aspartate Ammonia-Lyase
| Autor: | László Poppe, Diána Balogh-Weiser, Pál Csuka, Balázs Decsi, Evelin Sánta-Bell, Ali Obaid Imarah, Naran Bataa, Zsófia Molnár |
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| Rok vydání: | 2021 |
| Předmět: |
magnetic nanoparticles
Materials science Immobilized enzyme Mixing (process engineering) Nanoparticle 02 engineering and technology magnetic agitation lcsh:Chemical technology Aspartate ammonia-lyase 01 natural sciences Vial Catalysis lcsh:Chemistry lcsh:TP1-1185 aspartate ammonia-lyase Physical and Theoretical Chemistry enzyme immobilization 010405 organic chemistry Substrate (chemistry) equipment and supplies 021001 nanoscience & nanotechnology 0104 chemical sciences lcsh:QD1-999 Chemical engineering Magnet reactor design Magnetic nanoparticles 0210 nano-technology human activities |
| Zdroj: | Catalysts, Vol 11, Iss 483, p 483 (2021) Catalysts Volume 11 Issue 4 |
| ISSN: | 2073-4344 |
| Popis: | In this study, we investigated the influence of different modes of magnetic mixing on effective enzyme activity of aspartate ammonia-lyase from Pseudomonas fluorescens immobilized onto epoxy-functionalized magnetic nanoparticles by covalent binding (AAL-MNP). The effective specific enzyme activity of AAL-MNPs in traditional shake vial method was compared to the specific activity of the MNP-based biocatalyst in two devices designed for magnetic agitation. The first device agitated the AAL-MNPs by moving two permanent magnets at two opposite sides of a vial in x-axis direction (being perpendicular to the y-axis of the vial) the second device unsettled the MNP biocatalyst by rotating the two permanent magnets around the y-axis of the vial. In a traditional shake vial, the substrate and biocatalyst move in the same direction with the same pattern. In magnetic agitation modes, the MNPs responded differently to the external magnetic field of two permanent magnets. In the axial agitation mode, MNPs formed a moving cloud inside the vial, whereas in the rotating agitation mode, they formed a ring. Especially, the rotating agitation of the MNPs generated small fluid flow inside the vial enabling the mixing of the reaction mixture, leading to enhanced effective activity of AAL-MNPs compared to shake vial agitation. |
| Databáze: | OpenAIRE |
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