Description of a Natural Infection with Decapod Iridescent Virus 1 in Farmed Giant Freshwater Prawn, Macrobrachium rosenbergii
| Autor: | Jie Huang, Liang Qiu, Xing Chen, Chen Li, Wen Gao, Qingli Zhang, Ruo-Heng Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gill Veterinary medicine lcsh:QR1-502 Macrobrachium nipponense lcsh:Microbiology 03 medical and health sciences Virology CQIV Penaeus DIV1 Procambarus clarkii biology Macrobrachium rosenbergii susceptible species Hematopoietic Tissue white head 04 agricultural and veterinary sciences biology.organism_classification viral load 030104 developmental biology Infectious Diseases SHIV 040102 fisheries Prawn 0401 agriculture forestry and fisheries Hepatopancreas |
| Zdroj: | Viruses Volume 11 Issue 4 Viruses, Vol 11, Iss 4, p 354 (2019) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v11040354 |
| Popis: | Macrobrachium rosenbergii is a valuable freshwater prawn in Asian aquaculture. In recent years, a new symptom that was generally called &ldquo white head&rdquo has caused high mortality in M. rosenbergii farms in China. Samples of M. rosenbergii, M. nipponense, Procambarus clarkii, M. superbum, Penaeus vannamei, and Cladocera from a farm suffering from white head in Jiangsu Province were collected and analyzed in this study. Pathogen detection showed that all samples were positive for Decapod iridescent virus 1 (DIV1). Histopathological examination revealed dark eosinophilic inclusions and pyknosis in hematopoietic tissue, hepatopancreas, and gills of M. rosenbergii and M. nipponense. Blue signals of in situ digoxigenin-labeled loop-mediated isothermal amplification appeared in hematopoietic tissue, hemocytes, hepatopancreatic sinus, and antennal gland. Transmission electron microscopy of ultrathin sections showed a large number of DIV1 particles with a mean diameter about 157.9 nm. The virogenic stromata and budding virions were observed in hematopoietic cells. Quantitative detection with TaqMan probe based real-time PCR of different tissues in naturally infected M. rosenbergii showed that hematopoietic tissue contained the highest DIV1 load with a relative abundance of 25.4 ± 16.9%. Hepatopancreas and muscle contained the lowest DIV1 loads with relative abundances of 2.44 ± 1.24% and 2.44 ± 2.16%, respectively. The above results verified that DIV1 is the pathogen causing white head in M. rosenbergii. M. nipponense and Pr. clarkii are also species susceptible to DIV1. |
| Databáze: | OpenAIRE |
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