Breast adenocarcinoma-derived exosomes lower first-contact de-adhesion strength of adenocarcinoma cells to brain endothelial layer
| Autor: | Adrienn Fejér, Kinga Molnár, Attila Gergely Végh, Csilla Fazakas, Mihály Kozma, István A. Krizbai, András Dér, András Kincses, Imola Wilhelm, Barnabás Horváth |
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| Rok vydání: | 2020 |
| Předmět: |
Cell
02 engineering and technology Breast Adenocarcinoma Adenocarcinoma Exosomes Microscopy Atomic Force 01 natural sciences Exosome Metastasis Colloid and Surface Chemistry 0103 physical sciences Parenchyma medicine Cell Adhesion Humans Endothelium Physical and Theoretical Chemistry 010304 chemical physics Chemistry Brain Endothelial Cells Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology medicine.disease Microvesicles medicine.anatomical_structure Lymphatic system Cancer research 0210 nano-technology Biotechnology |
| Zdroj: | Colloids and surfaces. B, Biointerfaces. 204 |
| ISSN: | 1873-4367 |
| Popis: | Despite of advances in modern therapeutics, one of the most feared complications of cancer are brain metastases, which often cause life impairing profound neurological symptoms and premature death. Breast adenocarcinoma is among the leading "sources" of brain metastases. Since the central nervous system lacks a classical lymphatic circulation, invading metastatic cells can reach the brain parenchyma only through haematogenous routes and must breach the blood-brain barrier (BBB). The key step before the transmigration of metastatic cells through the highly regulated interface of the BBB is the establishment of firm adhesion between the tumor cell and the cerebral endothelial layer. Using atomic force microscopy, as a high resolution force spectrograph, direct measurements of intercellular interactions was performed between living adenocarcinoma cells and a confluent endothelial layer pre-treated with carcinoma cell-derived exosomes. By immobilization of a living adenocarcinoma cell to an atomic force microscope's cantilever, intercellular de-adhesions were directly measured by single cell force spectroscopy (SCFS) at quasi-physiological conditions. De-adhesion dynamics and strength was characterized by several different calculated parameters, involving aspects of both membrane and cell surface related factors. Our results indicate that de-adhesion strength was lower in case of exosome pre-treated endothelial cells as compared to non-treated controls. Breast adenocarcinoma-derived exosomes have direct effect on de-adhesion pattern of brain endothelium. |
| Databáze: | OpenAIRE |
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