Identification of Endothelial Cell Binding Sites on the Laminin γ1 Chain
| Autor: | Yoshihiko Yamada, Mucio C. Delgado, Hynda K. Kleinman, Motoyoshi Nomizu, Yuichiro Kuratomi, Katherine M. Malinda, Sharon K. Powell, Matthew P. Hoffman, M. Lourdes Ponce |
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| Rok vydání: | 1999 |
| Předmět: |
Endothelium
Physiology Angiogenesis Molecular Sequence Data Neovascularization Physiologic Chick Embryo Rats Sprague-Dawley Extracellular matrix Allantois Laminin Cell Adhesion medicine Animals Humans Amino Acid Sequence Binding site Aorta Basement membrane chemistry.chemical_classification Binding Sites biology Infant Newborn Chorion Rats Cell biology Endothelial stem cell medicine.anatomical_structure chemistry Immunology biology.protein Endothelium Vascular Peptides Cardiology and Cardiovascular Medicine Glycoprotein Cell Division |
| Zdroj: | Circulation Research. 84:688-694 |
| ISSN: | 1524-4571 0009-7330 |
| Popis: | Abstract —The laminins belong to a family of trimeric basement membrane glycoproteins with multiple domains, structures, and functions. Endothelial cells bind laminin-1 and form capillary-like structures when plated on a laminin-1–rich basement membrane matrix, Matrigel. Laminin-1 is composed of 3 chains, α1, β1, and γ1. Because laminin-1 is known to contain multiple biologically active sites, we have screened 156 synthetic overlapping peptides spanning the entire laminin γ1 chain for potential angiogenic sequences. Only 7 of these peptides, designated as C16, C25, C30, C38, C64, C75, and C102, disrupted the formation of capillary-like structures by human umbilical vein endothelial cells on Matrigel. Dose-response experiments in the presence of 50 to 200 μg/mL showed that tube formation was prevented by most peptides at 150 and 200 μg/mL, except for C16, which showed strong activity at all concentrations. Active peptides promoted vessel sprouting from aorta rings and angiogenesis in the chick chorioallantoic membrane assay. In addition, the active peptides also promoted endothelial cell adhesion to dishes coated with 0.1 μg of peptide and inhibited attachment to laminin-1 but not to plastic or fibronectin. Four of the active peptides, C25, C38, C75, and C102, may have cell-type specificity with endothelial cells, since they did not promote PC12 neurite outgrowth or adhesion of B16-F10 melanoma and human submandibular gland cells. These results suggest that specific laminin γ1–chain peptides have angiogenic activity with potential therapeutic applications. |
| Databáze: | OpenAIRE |
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