Instability of the cellular lipidome with age
| Autor: | Friedrich Leisch, Jessica R Hughes, Shane R. Ellis, Todd W. Mitchell, Stephen J. Blanksby, Roger J.W. Truscott, Jane M. Deeley |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male medicine.medical_specialty Ceramide Aging Spectrometry Mass Electrospray Ionization Adolescent Membrane lipids Phospholipid Glycerophospholipids Article Cataract Sampling Studies chemistry.chemical_compound Membrane Lipids Structure-Activity Relationship Young Adult Internal medicine medicine Humans Child Chromatography High Pressure Liquid Aged Aged 80 and over medicine.diagnostic_test Chemistry Age Factors Lens Nucleus Crystalline General Medicine Lipidome Middle Aged Sphingomyelins Endocrinology medicine.anatomical_structure Fiber cell Biochemistry Female Geriatrics and Gerontology Sphingomyelin Lipid profile Nucleus |
| Popis: | The human lens nucleus is formed in utero, and from birth onwards, there appears to be no significant turnover of intracellular proteins or membrane components. Since, in adults, this region also lacks active enzymes, it offers the opportunity to examine the intrinsic stability of macromolecules under physiological conditions. Fifty seven human lenses, ranging in age from 12 to 82 years, were dissected into nucleus and cortex, and the nuclear lipids analyzed by electrospray ionization tandem mass spectrometry. In the first four decades of life, glycerophospholipids (with the exception of lysophosphatidylethanolamines) declined rapidly, such that by age 40, their content became negligible. In contrast the level of ceramides and dihydroceramides, which were undetectable prior to age 30, increased approximately 100-fold. The concentration of sphingomyelins and dihydrosphingomyelins remained unchanged over the whole life span. As a consequence of this marked alteration in composition, the properties of fiber cell membranes in the centre of young lenses are likely to be very different from those in older lenses. Interestingly, the identification of age 40 years as a time of transition in the lipid composition of the nucleus coincides with previously reported macroscopic changes in lens properties (e.g., a massive age-related increase in lens stiffness) and related pathologies such as presbyopia. The underlying reasons for the dramatic change in the lipid profile of the human lens with age are not known, but are most likely linked to the stability of some membrane lipids in a physiological environment. |
| Databáze: | OpenAIRE |
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