A microRNA-152 that targets the phosphatase and tensin homolog to inhibit low oxygen induced-apoptosis in human brain microvascular endothelial cells
| Autor: | M Q Wang, L X Man, M Chi, Dianguo Li, Y H Cao, Binghe Xu, N Zhen, Y Y Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Phosphatase Apoptosis Rats Sprague-Dawley 03 medical and health sciences microRNA Genetics medicine Animals Humans Tensin PTEN Hypoxia Brain Molecular Biology biology HEK 293 cells PTEN Phosphohydrolase Brain Endothelial Cells General Medicine Transfection Hypoxia (medical) Molecular biology Cell Hypoxia Rats Cell biology Oxygen MicroRNAs HEK293 Cells 030104 developmental biology Microvessels Models Animal biology.protein medicine.symptom |
| Zdroj: | Genetics and Molecular Research. 15 |
| ISSN: | 1676-5680 |
| Popis: | Brain damage caused by perinatal asphyxia is dangerous for neonatal infants, but the mechanism by which it occurs remains elusive. In this study, microRNA-152 (miR-152) expression was induced by low oxygen levels in rat models of hypoxia brain damage, as well as in human brain microvascular endothelial cells (HBMECs) cultured in vitro. Analysis of the sequence of miR-152 revealed that the phosphatase and tensin homolog gene (PTEN) is probably the target of miR-152 both in humans and rats. When HBMECs were transfected with miR-152 mimics, PTEN expression was inhibited at both the mRNA and protein levels. Moreover, transfection with the miR-152 mimic also inhibited apoptosis induced by hypoxia. Furthermore, expression of the pro-apoptotic gene Bax was downregulated while the anti-apoptotic gene Bcl2 was upregulated after miR-152 mimic transfection. Taken together, these results indicate that miR-152 induced by hypoxia suppresses cell apoptosis and acts as a protective factor during hypoxia by repressing PTEN. |
| Databáze: | OpenAIRE |
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