Analysis of the Caenorhabditis elegans innate immune response to Coxiella burnetii
| Autor: | James M. Battisti, Myo T Naung, Lance A Watson, Adam M Drobish, Ekaterina Voronina, Michael F. Minnick |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hot Temperature MAP Kinase Kinase 4 030106 microbiology Immunology Mutant Q fever Nerve Tissue Proteins Biology Microbiology Article Animals Genetically Modified 03 medical and health sciences Gene Knockout Techniques Immunity medicine Animals Humans Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Innate immune system Obligate Wild type Cell Biology Coxiella burnetii biology.organism_classification medicine.disease bacterial infections and mycoses Immunity Innate Receptor Insulin Infectious Diseases bacteria Cattle Gram-Negative Bacterial Infections Q Fever Signal Transduction |
| Popis: | The nematode Caenorhabditis elegans is well established as a system for characterization and discovery of molecular mechanisms mediating microbe-specific inducible innate immune responses to human pathogens. Coxiella burnetii is an obligate intracellular bacterium that causes a flu-like syndrome in humans (Q fever), as well as abortions in domesticated livestock, worldwide. Initially, when wild type C. elegans (N2 strain) was exposed to mCherry-expressing C. burnetii (CCB) a number of overt pathological manifestations resulted, including intestinal distension, deformed anal region and a decreased lifespan. However, nematodes fed autoclave-killed CCB did not exhibit these symptoms. Although vertebrates detect C. burnetii via TLRs, pathologies in tol-1(–) mutant nematodes were indistinguishable from N2, and indicate nematodes do not employ this orthologue for detection of C. burnetii. sek-1(–) MAP kinase mutant nematodes succumbed to infection faster, suggesting that this signaling pathway plays a role in immune activation, as previously shown for orthologues in vertebrates during a C. burnetii infection. C. elegans daf-2(–) mutants are hyper-immune and exhibited significantly reduced pathological consequences during challenge. Collectively, these results demonstrate the utility of C. elegans for studying the innate immune response against C. burnetii and could lead to discovery of novel methods for prevention and treatment of disease in humans and livestock. |
| Databáze: | OpenAIRE |
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