CD44v3 and CD44v6 isoforms on T cells are able to discriminate different disease activity degrees and phenotypes in systemic lupus erythematosus patients
Autor: | Fulvia Ceccarelli, Guido Valesini, Francesca Romana Spinelli, Cristiana Barbati, Cristiano Alessandri, L. Novelli, Carlo Perricone, Fabrizio Conti, Roberto Perricone |
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Rok vydání: | 2019 |
Předmět: |
Adult
Male Genetic Markers 0301 basic medicine Gene isoform T-Lymphocytes Gene Expression Arthritis Severity of Illness Index Disease activity 03 medical and health sciences 0302 clinical medicine systemic lupus erythematosus Rheumatology nephritis medicine Lupus Erythematosus Systemic Humans arthritis biomarkers CD44v3/v6 disease activity Case-Control Studies Disease Progression Female Flow Cytometry Genetic Variation Hyaluronan Receptors Middle Aged Phenotype 030203 arthritis & rheumatology Lupus Erythematosus biology business.industry Systemic CD44 medicine.disease 030104 developmental biology Immunology biology.protein T cell migration business Nephritis Target organ |
Zdroj: | Lupus. 28:621-628 |
ISSN: | 1477-0962 0961-2033 |
Popis: | BackgroundAdhesion molecule CD44 contributes to T cell migration into target organs. A higher expression of CD44v3 and v6 isoforms has been identified on T cells from systemic lupus erythematosus (SLE) patients. The aim of this study was to investigate the expression of CD44v3/v6 on T cells of SLE patients in order to evaluate their correlation with clinical features.MethodsSixteen healthy subjects (HSs) and 33 SLE female patients were enrolled. Fifteen patients were in remission (Systemic Lupus Erythematosus Disease Activity Index-2000 (SLEDAI-2K) = 0) and 18 patients had an active disease (SLEDAI-2K ≥ 4). Experiments were conducted by flow cytometry.ResultsExpression of CD44v3 on CD4+and CD8+T cells was higher in active patients compared to HSs ( p = 0.0097 and p = 0.0096). CD44v3 on CD8+T cells was also higher in active patients compared to patients in remission ( p = 0.038). CD44v6 was higher on CD4+and CD8+T cells from active patients compared to HSs ( p = 0.003 and p = 0.0036) and to patients in remission ( p = 0.01 and p = 0.02). In active patients the ratio CD44v3/v6 was unbalanced towards isoform v6 on both T cell populations. In a receiver operating characteristic curve analysis, CD44v6 on CD4+T cells was the most sensitive and specific one (specificity of 81.8%, sensitivity of 75%). Expression of CD44v6 on CD4+and CD8+T cells correlated with the SLEDAI-2K ( p = 0.03, r = 0.38 and p = 0.02, r = 0.39). CD44v6 and CD44v3 on CD8+T cells associated with nephritis and arthritis ( p = 0.047 and p = 0.023).ConclusionsCD44v3/v6 can be used as biomarkers of disease activity and phenotypes; isoform v6 on CD4+T cells can be useful as a diagnostic biomarker. |
Databáze: | OpenAIRE |
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