Targeting Nanodiamonds to the Nucleus in Yeast Cells
| Autor: | Thamir Hamoh, Neda Norouzi, Emily P. P. Evans, Andreas Nagl, Aryan Morita, Alina Sigaeva, Kiran J. van der Laan, Romana Schirhagl |
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| Přispěvatelé: | Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE), Nanotechnology and Biophysics in Medicine (NANOBIOMED) |
| Rok vydání: | 2020 |
| Předmět: |
General Chemical Engineering
nucleus targeting 02 engineering and technology Article PROBES lcsh:Chemistry yeast cells Cell wall DELIVERY TRACKING 03 medical and health sciences medicine General Materials Science DIAMOND NANOPARTICLES 030304 developmental biology RELEASE 0303 health sciences fluorescence nanodiamonds Chemistry Spheroplast 021001 nanoscience & nanotechnology CANCER Yeast FLUORESCENT NANODIAMOND medicine.anatomical_structure lcsh:QD1-999 Drug delivery SPHEROPLASTS Biophysics Particle Diamond nanoparticles 0210 nano-technology Nucleus Intracellular |
| Zdroj: | Nanomaterials Volume 10 Issue 10 Nanomaterials, 10(10), 1-10. MDPI AG Nanomaterials, Vol 10, Iss 1962, p 1962 (2020) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10101962 |
| Popis: | Nanodiamonds are widely used for drug delivery, labelling or nanoscale sensing. For all these applications it is highly beneficial to have control over the intracellular location of the particles. For the first time, we have achieved targeting the nucleus of yeast cells. In terms of particle uptake, these cells are challenging due to their rigid cell wall. Thus, we used a spheroplasting protocol to remove the cell wall prior to uptake. To achieve nuclear targeting we used nanodiamonds, which were attached to antibodies. When using non-targeted particles, only 20% end up at the nucleus. In comparison, by using diamonds linked to antibodies, 70% of the diamond particles reach the nucleus. |
| Databáze: | OpenAIRE |
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