Protein kinase VRK-1 regulates cell invasion and EGL-17/FGF signaling in Caenorhabditis elegans
| Autor: | Paul W. Sternberg, Peter Askjaer, Pilar Alarcón, Valerie Reinke, Katherine Waters, Elke P. F. Klerkx |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Cell signaling
Somatic cell Recombinant Fusion Proteins vrk-1 Protein Serine-Threonine Kinases Article Vulva Cell polarity FGF Animals Transgenes Caenorhabditis elegans Caenorhabditis elegans Proteins Protein kinase A Molecular Biology Transcription factor biology urogenital system Uterus Gene Expression Regulation Developmental Cell Biology biology.organism_classification Receptors Fibroblast Growth Factor Molecular biology Cell invasion Cell biology Chromatin Fibroblast Growth Factors Anchor cell Intercellular Signaling Peptides and Proteins RNA Interference Vaccinia-related kinase Signal transduction Cell signalling Signal Transduction Developmental Biology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 11 pages, 6 figures.-- PMID: 19679119 [PubMed].-- Printed version published Nov 1, 2009. Supporting information available at: http://dx.doi.org/10.1016/j.ydbio.2009.08.007 The vaccinia-related kinases (VRKs) are highly conserved throughout the animal kingdom and phosphorylate several chromatin proteins and transcription factors. In early Caenorhabditis elegans embryos, VRK-1 is required for proper nuclear envelope formation. In this work, we present the first investigation of the developmental role of VRKs by means of a novel C. elegans vrk-1 mutant allele. We found that VRK-1 is essential in hermaphrodites for formation of the vulva, uterus, and utse and for development and maintenance of the somatic gonad and thus the germ line. VRK-1 regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage. VRK-1 is also required for proper specification and proliferation of uterine cells and sex myoblasts. Expression of the fibroblast growth factor-like protein EGL-17 and its receptor EGL-15 is reduced in vrk-1 mutants, suggesting that VRK-1 might act at least partially through activation of FGF signaling. Expression of a translational VRK-1::GFP fusion protein in the ventral nerve cord and vulva precursor cells restores vulva and uterus formation, suggesting both cell autonomous and non-autonomous roles of VRK-1. This work was funded by grants from the Spanish Ministry of Science (RYC-2003-001521, BFU-2004-01096, BFU-2007-60116) to PA. In addition, we wish to acknowledge EMBO and the Boehringer Ingelheim Foundation for support to EK and Junta de Andalucía for institutional support. PWS is an investigator with the Howard Hughes Medical Institute. Some nematode strains used in this work were provided by the International C. elegans Gene Knockout Consortium and the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources (NCRR). |
| Databáze: | OpenAIRE |
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