EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis
| Autor: | Antanas Planutis, James J. Bieker, Merlin Nithya Gnanapragasam, Kaustav Mukherjee, Andrew Chess, Li Xue |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Mouse QH301-705.5 Science Cell Kruppel-Like Transcription Factors Gene Expression KLF1 Biology General Biochemistry Genetics and Molecular Biology single cell sequencing 03 medical and health sciences 0302 clinical medicine transcription factors Gene expression EKLF/Klf1 medicine Transcriptional regulation Macrophage Biology (General) Transcription factor Gene General Immunology and Microbiology General Neuroscience Macrophages General Medicine Cell biology 030104 developmental biology medicine.anatomical_structure Single cell sequencing Erythropoiesis fetal liver island macrophage Medicine Function (biology) erythropoiesis 030215 immunology Research Article Developmental Biology |
| Zdroj: | eLife, Vol 10 (2021) eLife |
| Popis: | Erythroblastic islands are a specialized niche that contain a central macrophage surrounded by erythroid cells at various stages of maturation. However, identifying the precise genetic and transcriptional control mechanisms in the island macrophage remains difficult due to macrophage heterogeneity. Using unbiased global sequencing and directed genetic approaches focused on early mammalian development, we find that fetal liver macrophages exhibit a unique expression signature that differentiates them from erythroid and adult macrophage cells. The importance of erythroid Krüppel-like factor (EKLF)/KLF1 in this identity is shown by expression analyses in EKLF-/- and in EKLF-marked macrophage cells. Single-cell sequence analysis simplifies heterogeneity and identifies clusters of genes important for EKLF-dependent macrophage function and novel cell surface biomarkers. Remarkably, this singular set of macrophage island cells appears transiently during embryogenesis. Together, these studies provide a detailed perspective on the importance of EKLF in the establishment of the dynamic gene expression network within erythroblastic islands in the developing embryo and provide the means for their efficient isolation. |
| Databáze: | OpenAIRE |
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