Sperm Cohort-Specific Zinc Signature Acquisition and Capacitation-Induced Zinc Flux Regulate Sperm-Oviduct and Sperm-Zona Pellucida Interactions

Autor: Erma Z. Drobnis, Wei Xu, Miriam Sutovsky, Richard Oko, Nicolai V. Bovin, Karl Kerns, Mark R. Ellersieck, Momal Sharif, Lauren E. Hamilton, Peter Sutovsky, Michal Zigo, David J. Miller
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Swine
Oviducts
lcsh:Chemistry
0302 clinical medicine
Human fertilization
Zona pellucida
lcsh:QH301-705.5
Spectroscopy
reproductive and urinary physiology
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
Chemistry
zinc
General Medicine
Polyspermy
Spermatozoa
Computer Science Applications
Cell biology
medicine.anatomical_structure
Matrix Metalloproteinase 2
Female
Acrosome
Proteasome Endopeptidase Complex
endocrine system
capacitation
chemistry.chemical_element
Zinc
Semen analysis
sperm
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Capacitation
medicine
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
Zona Pellucida
Ion Transport
urogenital system
Organic Chemistry
oviductal reservoir
Sperm
Semen Analysis
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
fertilization
Oocytes
Cattle
Inner acrosomal membrane
Sperm Capacitation
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 6, p 2121 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 6
ISSN: 1422-0067
Popis: Building on our recent discovery of the zinc signature phenomenon present in boar, bull, and human spermatozoa, we have further characterized the role of zinc ions in the spermatozoa&rsquo
s pathway to fertilization. In boar, the zinc signature differed between the three major boar ejaculate fractions, the initial pre-rich, the sperm-rich, and the post-sperm-rich fraction. These differences set in the sperm ejaculatory sequence establish two major sperm cohorts with marked differences in their sperm capacitation progress. On the subcellular level, we show that the capacitation-induced Zn-ion efflux allows for sperm release from oviductal glycans as analyzed with the oviductal epithelium mimicking glycan binding assay. Sperm zinc efflux also activates zinc-containing enzymes and proteases involved in sperm penetration of the zona pellucida, such as the inner acrosomal membrane matrix metalloproteinase 2 (MMP2). Both MMP2 and the 26S proteasome showed severely reduced activity in the presence of zinc ions, through studies using by gel zymography and the fluorogenic substrates, respectively. In the context of the fertilization-induced oocyte zinc spark and the ensuing oocyte-issued polyspermy-blocking zinc shield, the inhibitory effect of zinc on sperm-borne enzymes may contribute to the fast block of polyspermy. Altogether, our findings establish a new paradigm on the role of zinc ions in sperm function and pave the way for the optimization of animal semen analysis, artificial insemination (AI), and human male-factor infertility diagnostics.
Databáze: OpenAIRE
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