Regulator of G-protein signaling (RGS) proteins differentially control chondrocyte differentiation
| Autor: | Frank Beier, C. Thomas G. Appleton, Claudine G. James |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Physiology
Cellular differentiation Clinical Biochemistry Gene Expression Cartilage metabolism Biology Chondrocyte RGS4 Mice Regulator of G protein signaling Chondrocytes Medicine and Health Sciences medicine Cyclic AMP Animals Collagen Type II RGS2 Cells Cultured Genetics fungi High Mobility Group Proteins Parathyroid Hormone-Related Protein Cell Differentiation SOX9 Transcription Factor Cell Biology Chondrogenesis Cell biology medicine.anatomical_structure Cartilage Gene Expression Regulation biology.protein sense organs RGS Proteins Signal Transduction Transcription Factors |
| Zdroj: | Bone and Joint Institute |
| ISSN: | 0021-9541 |
| Popis: | Control of chondrocyte differentiation is attained, in part, through G-protein signaling, but the functions of the RGS family of genes, well known to control G-protein signaling at the Gα subunit, have not been studied extensively in chondrogenesis. Recently, we have identified the Rgs2 gene as a regulator of chondrocyte differentiation. Here we extend these studies to additional Rgs genes. We demonstrate that the Rgs4, Rgs5, Rgs7, and Rgs10 genes are differentially regulated during chondrogenic differentiation in vitro and in vivo. To investigate the roles of RGS proteins during cartilage development, we overexpressed RGS4, RGS5, RGS7, and RGS10 in the chondrogenic cell line ATDC5. We found unique and overlapping effects of individual Rgs genes on numerous parameters of chondrocyte differentiation. In particular, RGS5, RGS7, and RGS10 promote and RGS4 inhibits chondrogenic differentiation. The identification of Rgs genes as novel regulators of chondrogenesis will contribute to a better understanding of both normal cartilage development and the etiology of chondrodysplasias and osteoarthritis. © 2006 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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