Role of Wnt/β-Catenin Pathway in the Arterial Medial Calcification and Its Effect on the OPG/RANKL System
| Autor: | Bin Nie, Siming Guan, Shaoqiong Zhou, Shao-ying Zhang, Xin Fang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
musculoskeletal diseases Myocytes Smooth Muscle Biochemistry Muscle Smooth Vascular Osteoprotegerin Genetics medicine Animals Humans Vascular Calcification Wnt Signaling Pathway Cells Cultured beta Catenin biology Chemistry RANK Ligand Wnt signaling pathway Cell Differentiation medicine.disease Molecular biology Rats Disease Models Animal Arterial calcification Gene Expression Regulation DKK1 RANKL Catenin Osteocalcin biology.protein Intercellular Signaling Peptides and Proteins Calcium Warfarin Lithium Chloride Calcification |
| Zdroj: | Current Medical Science. 39:28-36 |
| ISSN: | 2523-899X 2096-5230 |
| DOI: | 10.1007/s11596-019-1996-4 |
| Popis: | In this study, the hypothesis that Wnt/β-catenin pathway is involved in the arterial calcification by regulating the osteoprotegerin (OPG)/receptor activator of NF-κB ligand (RANKL) system was tested. The β-catenin expression was measured in the warfarin-induced calcified arteries and the osteoblast-like cells differentiating from smooth muscle cells (SMCs) by immunohistochemistry and Western blotting. The Wnt/β-catenin pathway was activated or inhibited by lithium chloride (LiCl) or dickkopf 1 (DKK1) in vitro and in vivo. Then the calcification level was determined by von Kossa staining, Ca2+ content assay, and alkaline phosphatase (ALP) activity assay. The expression levels of osteocalcin, OPG and RANKL were detected by Western blotting or real-time PCR. The results showed that in calcified arteries and OBL cells, the activation of Wnt/β-catenin pathway significantly enhanced the calcification as evidenced by increased von Kossa stains, Ca2+ contents, ALP activities, and osteocalcin expression levels (P |
| Databáze: | OpenAIRE |
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