Nanogram level quantitation of oxycodone in human plasma by capillary gas chromatography using nitrogen-phosphorus selective detection

Autor:	Patricia K. Padovani, Gilbert N. Lam, Shang-Ying P. King, Ram Kapil
Rok vydání:	1992
Předmět:	Male Chromatography Chromatography Gas Capillary action Chemistry Nitrogen Extraction (chemistry) Reproducibility of Results Phosphorus General Chemistry Plasma Toluene chemistry.chemical_compound Hydrocodone Oxycodone hydrochloride medicine Humans Gas chromatography Oxycodone medicine.drug
Zdroj:	Journal of chromatography. 577(2)
Popis:	A sensitive and specific capillary gas chromatographic assay is reported for the quantitation of oxycodone in human plasma. The technique involves a single extraction of oxycodone and internal standard (hydrocodone) from plasma by toluene containing 1% isopropanol. Separation is achieved on a methyl silicone (HP-1) fused-silica capillary column (25 m × 0.2 mm I.D., 0.33 μm film thickness) and detection is by nitrogen—phosphorus selective mode. The minimum quantifiable limit is 1.8 ng/ml using 2 ml of plasma. The method is applicable to characterize the plasma profile of oxycodone in humans after a single oral 5-mg oxycodone hydrochloride tablet.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37d322815274f71516f2ddc1720e36a1 https://pubmed.ncbi.nlm.nih.gov/1400759 Zobrazit plný text záznamu