The inhibitory roles of Ihh downregulation on chondrocyte growth and differentiation
| Autor: | Hongqi Zhang, Qile Gao, Minyu Hu, Yu-Xiang Wang, Shaohua Liu, Ang Deng, Chaofeng Guo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Indian hedgehog Chondrocyte hypertrophy Chondrocyte 03 medical and health sciences Immunology and Microbiology (miscellaneous) Downregulation and upregulation Osteoprotegerin endochondral bone formation medicine Bone growth biology Cell growth apoptosis Osteoblast Articles differentiation General Medicine biology.organism_classification body regions 030104 developmental biology medicine.anatomical_structure chondrocyte Cancer research cell cycle |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2017.5458 |
| Popis: | The proliferative rate of chondrocytes affects bone elongation. Chondrocyte hypertrophy is required for endochondral bone formation as chondrocytes secrete factors required for osteoblast differentiation and maturation. Previous studies have demonstrated that the Indian hedgehog (Ihh) signaling pathway is a key regulator of skeletal development and homeostasis. The aim of the present study was to investigate the function of Ihh in chondrocyte proliferation and differentiation, as well as the underlying mechanisms. Ihh was knocked down in mouse chondrocyte cells using short hairpin RNA. Chondrocyte apoptosis and cell cycle arrest were assessed using flow cytometry and the results indicated that knockdown of Ihh significantly inhibited cell growth (P |
| Databáze: | OpenAIRE |
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