Assessment of dentinal tubule invasion capacity ofEnterococcus faecalisunder stress conditionsex vivo
| Autor: | Wei Jiang, Cai-lian Zhu, J P Liang, Shujun Ran, Juan Wang |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Microscopy
Confocal Biofilm Smear layer In Vitro Techniques Biology biology.organism_classification Molecular biology Enterococcus faecalis Tryptic soy broth Microbiology chemistry.chemical_compound Tubule Dentinal Tubule chemistry Stress Physiological Biofilms Dentin Humans General Dentistry Incubation Ex vivo |
| Zdroj: | International Endodontic Journal. 48:362-372 |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12322 |
| Popis: | Aim To investigate the dentinal tubule invasion capacity of Enterococcus faecalis under alkaline and energy starvation stress conditions. Methodology The root canals from human single-rooted teeth (n = 40) were infected with E. faecalis under alkaline (pH 9, 10, 11 and 12) and energy starvation (no glucose, 0.05% glucose and 0.15% glucose) stress conditions. The root canals were prepared in a standard manner and treated to remove the smear layer before incubation. After 4 weeks of cultivation, the roots were split vertically into two halves: one half was processed for biofilm formation analysis using a scanning electron microscope; the other half was stained with fluorescent DNA-binding reagents, washed thoroughly and sectioned (100 μm thick), and the depth of tubule invasion by the microorganism was examined by confocal laser-scanning microscopy. The extent of dentine tubule invasion was analysed statistically. Results The E. faecalis strain resulted in biofilm formation and dentine tubules invasion under all of the stress conditions, except for pH 11 and 12 conditions. However, the tubule penetration distance was markedly reduced in these stress conditions (P |
| Databáze: | OpenAIRE |
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