Multicenter Study of Method-Dependent Epidemiological Cutoff Values for Detection of Resistance in Candida spp. and Aspergillus spp. to Amphotericin B and Echinocandins for the Etest Agar Diffusion Method
| Autor: | Nelesh P. Govender, S. Cordoba, Guillermo Garcia-Effron, Maurizio Sanguinetti, Maiken Cavling Arendrup, Teresa Peláez, John D. Turnidge, Jesús Guinea, Ana Espinel-Ingroff, Ryan K. Shields, Cornelia Lass-Flörl, Michaela Lackner, Estrella Martín-Mazuelos, Gloria M. González, Manuel Rodríguez-Iglesias, Eric Dannaoui, Julio García-Rodríguez, Emilia Cantón, M. J. Linares Sicilia |
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| Přispěvatelé: | [Espinel-Ingroff, A.] VCU, Med Ctr, Richmond, VA 23284 USA, [Arendrup, M.] Statens Serum Inst, Unit Mycol, Copenhagen, Denmark, [Arendrup, M.] Univ Copenhagen, Rigshosp, Copenhagen, Denmark, [Canton, E.] Inst Invest Sanitaria La Fe, Grp Infecc Grave, Valencia, Spain, [Cordoba, S.] Inst Nacl Enfermedades Infecciosas Dr CG Malbran, Buenos Aires, DF, Argentina, [Dannaoui, E.] Univ Paris 05, Hop Europeen Georges Pompidou, AP HP, Fac Med,Unite Parasitol Mycol,Serv Microbiol, Paris, France, [Garcia-Rodriguez, J.] Hosp Univ La Paz, Madrid, Spain, [Gonzalez, G. M.] Univ Autonoma Nuevo Leon, Monterrey, Nuevo Leon, Mexico, [Govender, N. P.] Natl Inst Communicable Dis, Johannesburg, South Africa, [Martin-Mazuelos, E.] Hosp Univ Valme, Unidad Gest Clin Enfermedades Infecciosas & Micro, Seville, Spain, [Lackner, M.] Med Univ Innsbruck, Div Hyg & Med Microbiol, Innsbruck, Austria, [Lass-Florl, C.] Med Univ Innsbruck, Div Hyg & Med Microbiol, Innsbruck, Austria, [Linares Sicilia, M. J.] Univ Cordoba, Hosp Univ Reina Sofia, Fac Med, Cordoba, Spain, [Rodriguez-Iglesias, M. A.] Hosp Univ Puerta del Mar, Cadiz, Spain, [Pelaez, T.] Hosp Univ Cent Asturias, Serv Microbiol, Asturias, Spain, [Shields, R. K.] Univ Pittsburgh, Med Ctr, Pittsburgh, PA USA, [Garcia-Effron, G.] Univ Nacl Litoral, Lab Micol & Diagnost Mol, Fac Bioquim & Ciencias Biol, CONICET,CCT, Santa Fe, Argentina, [Guinea, J.] Hosp Gen Univ Gregorio Maranon, Serv Microbiol Clin & Enfermedades Infecciosas VI, Madrid, Spain, [Guinea, J.] Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain, [Sanguinetti, M.] Univ Cattolica Sacro Cuore, Inst Microbiol, Rome, Italy, [Sanguinetti, M.] Univ Cattolica Sacro Cuore, Inst Hyg, Rome, Italy, [Turnidger, J.] Univ Adelaide, Adelaide, SA, Australia, NIAID NIH HHS, NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Echinocandin resistance Isolates causing fungemia Antifungal Agents WT isolates Eucast technical note Anidulafungin Aspergillus fumigatus chemistry.chemical_compound Amphotericin B resistance Echinocandins South Africa Disk Diffusion Antimicrobial Tests Amphotericin B Pharmacology (medical) Clsi Candida albicans skin and connective tissue diseases Candida Caspofungin mics education.field_of_study Mic distributions biology antifungal resistance Europe Infectious Diseases Aspergillus Etest MICs Candida Sensititre yeastone echinocandin resistance Broth microdilution medicine.drug Echinocandin 030106 microbiology Population Non-WT Method m27-a3 document Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA Microbiology Etest ECVs Susceptibility marker 03 medical and health sciences Antifungal resistance ECVs Etest MICs Aspergillus Pharmacology susceptibility marker Drug Resistance Fungal medicine education Etest biochemical phenomena metabolism and nutrition amphotericin B resistance biology.organism_classification bacterial infections and mycoses United States Latin America chemistry Susceptibility Caspofungin non-WT Antifungal susceptibility |
| Zdroj: | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid |
| ISSN: | 0066-4804 |
| Popis: | Method-dependent Etest epidemiological cutoff values (ECVs) are not available for susceptibility testing of either Candida or Aspergillus species with amphotericin B or echinocandins. In addition, reference caspofungin MICs for Candida spp. are unreliable. Candida and Aspergillus species wild-type (WT) Etest MIC distributions (microorganisms in a species-drug combination with no detectable phenotypic resistance) were established for 4,341 Candida albicans , 113 C. dubliniensis , 1,683 C. glabrata species complex (SC), 709 C. krusei , 767 C. parapsilosis SC, 796 C. tropicalis , 1,637 Aspergillus fumigatus SC, 238 A. flavus SC, 321 A. niger SC, and 247 A. terreus SC isolates. Etest MICs from 15 laboratories (in Argentina, Europe, Mexico, South Africa, and the United States) were pooled to establish Etest ECVs. Anidulafungin, caspofungin, micafungin, and amphotericin B ECVs (in micrograms per milliliter) encompassing ≥97.5% of the statistically modeled population were 0.016, 0.5, 0.03, and 1 for C. albicans ; 0.03, 1, 0.03, and 2 for C. glabrata SC; 0.06, 1, 0.25, and 4 for C. krusei ; 8, 4, 2, and 2 for C. parapsilosis SC; and 0.03, 1, 0.12, and 2 for C. tropicalis . The amphotericin B ECV was 0.25 μg/ml for C. dubliniensis and 2, 8, 2, and 16 μg/ml for the complexes of A. fumigatus , A. flavus , A. niger , and A. terreus , respectively. While anidulafungin Etest ECVs classified 92% of the Candida fks mutants evaluated as non-WT, the performance was lower for caspofungin (75%) and micafungin (84%) cutoffs. Finally, although anidulafungin (as an echinocandin surrogate susceptibility marker) and amphotericin B ECVs should identify Candida and Aspergillus isolates with reduced susceptibility to these agents using the Etest, these ECVs will not categorize a fungal isolate as susceptible or resistant, as breakpoints do. |
| Databáze: | OpenAIRE |
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