Multi-mycotoxin LC-MS/MS method validation and its application to fifty-four wheat flours in Hungary
| Autor: | Csilla Sörös, Péter Fodor, Emese Varga |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis Flour Wheat flour Food Contamination Biology Toxicology 01 natural sciences chemistry.chemical_compound 0404 agricultural biotechnology Tandem Mass Spectrometry Lc ms ms Mycotoxin Chromatography High Pressure Liquid Triticum Hungary Chromatography 010401 analytical chemistry Public Health Environmental and Occupational Health 04 agricultural and veterinary sciences General Chemistry General Medicine Mycotoxins 040401 food science 0104 chemical sciences chemistry Hplc ms ms Food Analysis Food Science Multi mycotoxin |
| DOI: | 10.6084/m9.figshare.13873713.v1 |
| Popis: | In this study, fifty-four wheat flour and wheat-based products available on the Hungarian market were assessed for twelve mycotoxins. Prior to analysis, a multi-mycotoxin method using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed and validated for wheat and wheat-based products. A simple extraction with acetonitrile/water/formic acid (79/20/1 v/v%) was used for sample preparation. The limits of quantitation (LOQ) were between 0.02 and 161 μg kg−1. Good linearity (r2 > 0.995) was achieved for all mycotoxins investigated. Recoveries varied between 88 and 120% at three concentration levels. Based on the low relative matrix effect (RSD < 0.15%) of the different wheat flour samples, matrix-matched calibration was used, which also proved its suitability in proficiency testing (z-scores: −0.6 for DON; +1.5 for OTA; −0.5 for ZEA). DON was the predominant mycotoxin, which contaminated 84% of the investigated samples. Metabolised forms of DON were found in spelt, durum flour and some wheat-based products (D3G in 4 samples, 15Ac-DON in 7 samples). T-2 and HT-2 were the second most frequently detected mycotoxins. All investigated samples complied with current European/Hungarian legislation. |
| Databáze: | OpenAIRE |
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