Reversed phase liquid chromatography for the enantioseparation of local anaesthetics in polysaccharide-based stationary phases. Application to biodegradability studies
| Autor: | Laura Escuder-Gilabert, María José Medina-Hernández, Mireia Perez-Baeza, Salvador Sagrado, Yolanda Martín-Biosca |
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| Rok vydání: | 2020 |
| Předmět: |
Acetonitriles
Resolution (mass spectrometry) Mepivacaine 010402 general chemistry 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Reversed phase conditions Polysaccharides Phase (matter) medicine Enantioselective biodegradation study Anesthetics Local Acetonitrile Local anaesthetics Chromatography High Pressure Liquid Chromatography Reverse-Phase Aqueous solution Chromatography Cellulose and amylose-based chiral stationary phases Methanol 010401 analytical chemistry Organic Chemistry Enantioselective synthesis Water Stereoisomerism General Medicine Reversed-phase chromatography 0104 chemical sciences Molecular Weight Biodegradation Environmental chemistry Enantiomer medicine.drug |
| Zdroj: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| ISSN: | 1873-3778 |
| Popis: | [EN] A comprehensive study on the chiral separation of bupivacaine, mepivacaine, prilocaine and propanocaine with eight commercial polysaccharide-based chiral stationary phases (CSPs) in reversed phase conditions compatible with MS detection is performed. Methanol and acetonitrile are used as organic modifiers. Retention and resolution values obtained for each compound in the different CSPs and mobile phases are compared. The polysaccharide-based CSPs tested present different enantioselectivity towards the analytes. From the results, the experimental conditions for determining the enantiomers of bupivacaine, mepivacaine, prilocaine and propanocaine in saline aqueous samples using MS detection are used, for the first time, to perform an enantioselective biodegradability study. (C) 2020 Elsevier B.V. All rights reserved. The authors acknowledge the Spanish Ministerio de Economia y Competitividad (MINECO) and the European Regional Development Fund (ERDF) for the financial support (Project CTQ2015-70904-R, MINECO/FEDER, UE). Mireia Perez Baeza acknowledges to Generalitat Valenciana and European Social Fund for the contribution to the contract ACIF/2019/158 |
| Databáze: | OpenAIRE |
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