QiShenYiQi Pills® prevent cardiac ischemia-reperfusion injury via energy modulation
| Autor: | Bai-He Hu, Jing-Yan Han, Chaun-She Wang, Chun-Shui Pan, Xiao-Hong Wei, Yu-Ying Liu, Ping Huang, Se-Qi Lin, Hong-Ning Liu, Li Quan, Xin Chang, Xiaoyuan Yang, Na Zhao, Jing-Yu Fan |
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| Rok vydání: | 2012 |
| Předmět: |
Cardiac function curve
Male medicine.medical_specialty Cardiotonic Agents Ischemia Myocardial Reperfusion Injury Pharmacology ATP5D Rats Sprague-Dawley Medicine Animals Myocardial infarction ATP synthase biology business.industry medicine.disease Surgery Rats Apoptosis biology.protein Cardiology and Cardiovascular Medicine business Energy Metabolism Reperfusion injury Drugs Chinese Herbal |
| Zdroj: | International journal of cardiology. 168(2) |
| ISSN: | 1874-1754 |
| Popis: | Background QiShenYiQi Pills® (QSYQ) is a compound Chinese medicine used in China for alleviating cardiac function. The present study was designed to explore the effect and mechanism of QSYQ on ischemia–reperfusion (I/R)-induced disorders in myocardial structure and function, with particularly focusing on the regulation of energy metabolism. Methods Sprague–Dawley rats, with or without QSYQ pretreatment, were subjected to 30min occlusion of the left anterior descending coronary artery and followed by 90min or 24h reperfusion. Myocardial blood flow (MBF) and cardiac function were evaluated at baseline, immediately after ischemia and 30, 60, 90min, and 24h after reperfusion. Myocardial infarction, myocardial histology and ultrastructure were assessed. Double staining of alpha-cardiac actinin and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was conducted to assess myocardial apoptosis. ATP, ADP and AMP content was determined by Enzyme-Linked Immunosorbent Assay, F-actin in myocardial cells determined by immunofluorescence microscopy and expression of ATP synthase α, ATP5D, and phosphorylated-Myosin Light Chain (P-MLC) determined by western blotting. Results Pre-treatment with QSYQ protected against I/R-induced MBF decrease, myocardial infarction and apoptosis at 90min and 24h after reperfusion. Moreover, I/R 90min caused an impairment on cardiac function, a decrease in the ratio of ADP/ATP and AMP/ATP, accompanying with reduction of ATP 5D expression and increase in the expression of P-MLC, meanwhile, myocardium to exhibit myocardial fiber rupture, interstitial edema, and mitochondria swelling, all of which were significantly ameliorated by pre-treatment with QSYQ. Conclusions The results of the present study suggest an involvement of regulation of energy metabolism in the action of QSYQ to protect against myocardial I/R injury. |
| Databáze: | OpenAIRE |
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