Actin-related protein 4: An unconventional negative regulator of mitochondrial calcium in protozoan parasite Leishmania
| Autor: | Ranju Bajpai, Niranjan Kumar Veluru, Aastha Singh, Kalyan Mitra, Amogh A. Sahasrabuddhe, Lova Prasadareddy Kajuluri |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Leishmania
biology Antibodies Protozoan chemistry.chemical_element Cell Biology Mitochondrion Calcium Pyruvate dehydrogenase complex biology.organism_classification Calcium uptake Protozoan parasite Actins Mitochondria Negative regulator Cell biology Gene Expression Regulation chemistry parasitic diseases Animals Molecular Medicine Rabbits Molecular Biology Actin |
| Zdroj: | Mitochondrion. 62:31-40 |
| ISSN: | 1567-7249 |
| Popis: | Regulation of mitochondrial calcium import is less understood in evolutionarily distinct protozoan parasites, such as Leishmania, as some of the mitochondrial calcium uniporter complex proteins are either missing or functionally diverged. Here, we show that Actin-related protein4 (ARP4), localizes exclusively into the Leishmania mitochondrion and depletion of this protein causes cells to accumulate calcium in the mitochondrion. The ARP4 depleted cells show increased activation of pyruvate dehydrogenase and production of ATP. Overall, our results indicate that ARP4 negatively regulates calcium uptake in the Leishmania mitochondrion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect