Microgenomics of ameloblastoma
| Autor: | Darrin Simmons, V. Murrah, Cynthia Suggs, Patricia DeVilliers, John Tim Wright |
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| Rok vydání: | 2011 |
| Předmět: |
Patched Receptors
Pathology medicine.medical_specialty Microarray Receptors Cell Surface Biology Receptors G-Protein-Coupled Ameloblastoma S100 Calcium Binding Protein G Dental Enamel Proteins Gene expression medicine Biomarkers Tumor Humans Hedgehog Proteins RNA Messenger RNA Neoplasm General Dentistry Gene Microdissection Laser capture microdissection Oligonucleotide Array Sequence Analysis Extracellular Matrix Proteins Amelogenin Gene Expression Profiling Nucleic Acid Hybridization Proteins Research Reports Genomics medicine.disease Molecular biology Smoothened Receptor Neoplasm Proteins Gene expression profiling Wnt Proteins Matrix Metalloproteinase 20 Calbindin 2 Significance analysis of microarrays Kallikreins Lasers Semiconductor |
| Zdroj: | Journal of dental research. 90(4) |
| ISSN: | 1544-0591 |
| Popis: | Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets. |
| Databáze: | OpenAIRE |
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