Implanted Dental Pulp Cells Fail to Induce Regeneration in Partial Pulpotomies
| Autor: | Francesca Mangione, Claire Bardet, M Bonneau, Franck Decup, Mostafa EzEldeen, Reinhilde Jacobs, S. Opsahl-Vital, Julie Lesieur, Catherine Chaussain, Benjamin Salmon |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Molar Bone sialoprotein Swine Sialoglycoproteins Pulpotomy Dentistry Dentin Secondary Masson's trichrome stain 03 medical and health sciences 0302 clinical medicine stomatognathic system Tissue engineering Dentin medicine Animals Integrin-Binding Sialoprotein Regeneration General Dentistry Dental Pulp Cell Proliferation Extracellular Matrix Proteins Staining and Labeling Tissue Engineering biology Chemistry business.industry Hydrogels X-Ray Microtomography 030206 dentistry Phosphoproteins stomatognathic diseases 030104 developmental biology medicine.anatomical_structure biology.protein Swine Miniature Pulp (tooth) business Dentin sialoprotein |
| Zdroj: | Journal of Dental Research. 96:1406-1413 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | Cell-based partial pulp regeneration is one of the promising approaches to obtain newly formed functional dentin-pulp complex. It relies on the preservation of the healthy tissue while regenerating the damaged pulp. The aim of this study was to investigate whether this regenerative process could be achieved by implanting porcine dental pulp cells (pDPCs) in pulp defects in the minipig. By split-mouth model, self-assembling injectable nanopeptide hydrogel, with and without pDPCs, was implanted after cameral pulpotomy in premolars and molars. At day 21 after surgery, 3-dimensional morphometric characterization, Masson's trichrome staining, and immunolabeling for DSP and BSP (dentin sialoprotein and bone sialoprotein) were performed on treated teeth. This study demonstrated no pulp regeneration but systematic reparative dentinogenesis. In fact, regardless of the presence of pDPCs in the scaffold, an osteodentin bridge-the microarchitecture of which significantly differed from the native dentin-was systematically obtained. Furthermore, the presence of pDPCs significantly affected the microstructure of the dentin bridges. In the radicular area of each treated tooth, hyperemia in the remaining pulp and external root resorptions were observed. Under the conditions tested in this work, pulp regeneration was not achieved, which highlights the need of further investigations to develop favorable regenerative microenvironment. |
| Databáze: | OpenAIRE |
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