The anti-inflammatory and anti-apoptotic effects of nesfatin-1 in the traumatic rat brain
| Autor: | Hong-Song Pan, Xiao-Jun Fu, Chonghui Tang, Xiao-Jie Wei, Xin-Long Xu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Pathology Subarachnoid hemorrhage Physiology medicine.drug_class Traumatic brain injury Gene Expression Apoptosis Nerve Tissue Proteins Inflammation Biochemistry Anti-inflammatory Head trauma Cellular and Molecular Neuroscience Endocrinology Internal medicine Gene expression medicine Animals Nucleobindins Rats Wistar Neurons Caspase 3 business.industry Calcium-Binding Proteins NF-kappa B medicine.disease Rats DNA-Binding Proteins Brain Injuries Cytokines Tumor necrosis factor alpha Inflammation Mediators medicine.symptom business Injections Intraperitoneal |
| Zdroj: | Peptides. 36:39-45 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2012.04.014 |
| Popis: | Nesfatin-1 has been demonstrated to possess anti-inflammatory and anti-apoptotic effects in the rat brain with subarachnoid hemorrhage. The study was designed to investigate the influence of nesfatin-1 on inflammatory responses and neuronal cell apoptosis after traumatic brain injury. Wistar rats were subjected to 5, 10 or 20 μg/kg of nesfatin-1 at designed time points (0.5, 2, 4 or 8h after head trauma) intraperitoneally. Rats were sacrificed at hours 2, 6 and 12, as well as day 1, 2, 3 and 5 after head trauma. The administration of 10 or 20 μg/kg of nesfatin-1 at hour 0.5 after head trauma could significantly suppress gene expressions of nuclear factor kappa-B, lessen concentrations of tumor necrosis factor-alpha, interleukin-1beta and interleukin-6, diminish caspase-3 activity as well as reduce number of apoptotic neuronal cells in traumatic rat brain tissues (P0.05), but the administration of 5 μg/kg of nesfatin-1 not (P0.05). Moreover, 20 μg/kg nesfatin-1 also significantly suppressed the inflammation and neuronal cell apoptosis when applied 2, 4 or 8h after head trauma. However, a clear concentration-response or time-response relationship was not found. These findings suggest that nesfatin-1 may inhibit nuclear factor kappa-B-dependent inflammatory responses, and lessen caspase-3-mediated neuronal cell apoptosis after traumatic brain injury in rats. |
| Databáze: | OpenAIRE |
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