Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency
| Autor: | Wenfeng Qian, Xiu-Li Hou, Qing Huan, Chun-Ming Liu, Yi Zhang, Ian M. Silverman, Brian D. Gregory, Taolan Zhao, Xiaofeng Cao, Jing Sun, Xiang Yu, Chunyan Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Polyadenylation
Translational efficiency lcsh:QH426-470 Arabidopsis Guanosine Genes Plant Poly(A)-Binding Protein II Poly(A) tails 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gene Expression Regulation Plant Poly(A)-binding protein RNA Messenger mRNA stability Poly(A)-tail G-content Gene lcsh:QH301-705.5 030304 developmental biology Regulation of gene expression Base Composition 0303 health sciences Messenger RNA biology Arabidopsis Proteins Research biology.organism_classification Poly(A)-binding proteins Cell biology lcsh:Genetics chemistry lcsh:Biology (General) Protein Biosynthesis biology.protein 030217 neurology & neurosurgery PAB binding efficiency Protein Binding |
| Zdroj: | Genome Biology, Vol 20, Iss 1, Pp 1-12 (2019) Genome Biology |
| DOI: | 10.1186/s13059-019-1799-8 |
| Popis: | Background Polyadenylation plays a key role in producing mature mRNAs in eukaryotes. It is widely believed that the poly(A)-binding proteins (PABs) uniformly bind to poly(A)-tailed mRNAs, regulating their stability and translational efficiency. Results We observe that the homozygous triple mutant of broadly expressed Arabidopsis thaliana PABs, AtPAB2, AtPAB4, and AtPAB8, is embryonic lethal. To understand the molecular basis, we characterize the RNA-binding landscape of these PABs. The AtPAB-binding efficiency varies over one order of magnitude among genes. To identify the sequences accounting for the variation, we perform poly(A)-seq that directly sequences the full-length poly(A) tails. More than 10% of poly(A) tails contain at least one guanosine (G); among them, the G-content varies from 0.8 to 28%. These guanosines frequently divide poly(A) tails into interspersed A-tracts and therefore cause the variation in the AtPAB-binding efficiency among genes. Ribo-seq and genome-wide RNA stability assays show that AtPAB-binding efficiency of a gene is positively correlated with translational efficiency rather than mRNA stability. Consistently, genes with stronger AtPAB binding exhibit a greater reduction in translational efficiency when AtPAB is depleted. Conclusions Our study provides a new mechanism that translational efficiency of a gene can be regulated through the G-content-dependent PAB binding, paving the way for a better understanding of poly(A) tail-associated regulation of gene expression. Electronic supplementary material The online version of this article (10.1186/s13059-019-1799-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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