Tong-Xie-Yao-Fang improves intestinal permeability in diarrhoea-predominant irritable bowel syndrome rats by inhibiting the NF-κB and notch signalling pathways

Autor: Liu Liao, Zhaoyang Zhu, Yongquan Huang, Xinlin Chen, Qiuke Hou, Quanbin Han, Fengbin Liu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Diarrhea
Male
Chemokine
H&E stain
Notch signaling pathway
Granulocyte
Pharmacology
Intestinal permeability
Tong-Xie-Yao-fang
Diarrhoea predominant-irritable bowel syndrome
Irritable Bowel Syndrome
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
medicine
Animals
Intestinal Mucosa
Irritable bowel syndrome
Receptors
Notch
biology
medicine.diagnostic_test
business.industry
Notch Signalling
NF-kappa B
General Medicine
lcsh:Other systems of medicine
medicine.disease
lcsh:RZ201-999
Rats
Rifaximin
Disease Models
Animal
medicine.anatomical_structure
Intestinal Absorption
Complementary and alternative medicine
chemistry
NF-κB Signalling
030220 oncology & carcinogenesis
biology.protein
Cytokines
Female
030211 gastroenterology & hepatology
business
Research Article
Drugs
Chinese Herbal
Signal Transduction
Zdroj: BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-10 (2019)
BMC Complementary and Alternative Medicine
ISSN: 1472-6882
DOI: 10.1186/s12906-019-2749-4
Popis: Background Tong-Xie-Yao-Fang (TXYF) has been shown to be effective in diarrhoea-predominant irritable bowel syndrome (IBS-D) patients. However, the underlying mechanism remains to be clarified. The aim of this study was to investigate the efficacy and related mechanisms of TXYF in an IBS-D rat model. Methods The IBS-D rat model was established with 4% acetic acid and evaluated by haematoxylin-eosin (HE) staining. Then, IBS-D rats were divided into control, TXYF and rifaximin groups and treated intragastrically with normal saline, TXYF and rifaximin, respectively, for 14 days. The following indicators were measured before and after treatment: defecation frequency, faecal water content (FWC) and colorectal distension (CRD). Histopathological changes in the distal colon were observed after treatment. The expression of OCLN and ZO1 in the distal colon of IBS-D rats reflected the intestinal mucosal permeability, as measured by qRT-PCR, western blot, and enzyme-linked immunosorbent assays (ELISAs). The NF-κB and Notch signalling pathways and inflammation-related factors were investigated. Results After treatment with TXYF, the defecation frequency, FWC and CRD were significantly lower than those in the model group (P P P P Conclusion TXYF can effectively improve intestinal permeability and enhance intestinal mucosal barrier function, which may be related to inhibition of the inflammatory cascade and the NF-κB and Notch signalling pathways.
Databáze: OpenAIRE
Externí odkaz: