Formulation and optimization of celecoxib-loaded PLGA nanoparticles by the Taguchi design and theirin vitrocytotoxicity for lung cancer therapy

Autor:	Jaleh Varshosaz, Hojat Sadeghi, Aida Pourmashhadi, Hamed Hamishehkar, Jaber Emami
Rok vydání:	2014
Předmět:	Pharmaceutical Science Nanoparticle Nanotechnology General Medicine Solvent PLGA chemistry.chemical_compound Pulmonary surfactant chemistry Emulsion Zeta potential Celecoxib medicine Geometric standard deviation Nuclear chemistry medicine.drug
Zdroj:	Pharmaceutical Development and Technology. 20:791-800
ISSN:	1097-9867 1083-7450
DOI:	10.3109/10837450.2014.920360
Popis:	The objective of the present study was to develop, evaluate and optimize a polymeric nanoparticle (NP) system containing Cxb for pulmonary delivery of Cxb in the treatment of lung cancer. NPs were prepared by the emulsion solvent diffusion and evaporation method using poly(D, L lactideglycolide) (PLGA). The size of NPs ranged from 153 to 192 nm and was affected by PLGA content, surfactant concentration and organic phase volume. Zeta potential of NPs (-4.5 to -8.6 mV) was more affected by PLGA content and organic phase volume. PLGA content was also the most effective factor on the entrapment efficiency and release rate of Cxb from NPs. The optimum formulation which obtained with 5 mg Cxb, 25 mg PLGA, 0.5% surfactant, 2.5% organic volume and 15 000 rpm showed release of Cxb within 30 h. The optimized formulation co-spray dried with lactose (hybrid microparticles) displayed desirable fine particle fraction, mass medium aerodynamic diameter, geometric standard deviation of 70.3%, 1.46% and 3.38%, respectively. Our results provide evidence for the potential of PLGA NPs for delivery of Cxb through inhalation as means to alleviate the cardiovascular risk of Cxb administration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37babe7d3cabcffef1448912ade37c03 https://doi.org/10.3109/10837450.2014.920360 Zobrazit plný text záznamu