Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine
| Autor: | Yusuf Yagci, Senem Kiralp, Levent Toppare, A. Elif Böyükbayram |
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| Rok vydání: | 2006 |
| Předmět: |
Immobilized enzyme
Polymers Supporting electrolyte Biophysics Wine Biosensing Techniques Electrochemistry Polyphenol oxidase chemistry.chemical_compound Phenols Thiophene Polytetrahydrofuran Organic chemistry Pyrroles Physical and Theoretical Chemistry Fourier transform infrared spectroscopy Furans Catechol oxidase biology Electric Conductivity Equipment Design General Medicine Enzymes Immobilized Equipment Failure Analysis chemistry biology.protein Catechol Oxidase Food Analysis Nuclear chemistry |
| Zdroj: | Bioelectrochemistry. 69:164-171 |
| ISSN: | 1567-5394 |
| DOI: | 10.1016/j.bioelechem.2006.01.006 |
| Popis: | Electrochemically produced graft copolymers of thiophene capped polytetrahydofuran (TPTHF1 and TPTHF2) and pyrrole were achieved by constant potential electrolysis using sodium dodecylsulfate (SDS) as the supporting electrolyte. Characterizations were based on Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Electrical conductivities were measured by the four-probe technique. Novel biosensors for phenolic compounds were constructed by immobilizing polyphenol oxidase (PPO) into conducting copolymers prepared by electropolymerization of pyrrole with thiophene capped polytetrahydrofuran. Kinetic parameters, maximum reaction rate (V(max)) and Michaelis-Menten constant (K(m)) and optimum conditions regarding temperature and pH were determined for the immobilized enzyme. Operational stability and shelf-life of the enzyme electrodes were investigated. Enzyme electrodes of polyphenol oxidase were used to determine the amount of phenolic compounds in two brands of Turkish red wines and found very useful owing to their high kinetic parameters and wide pH working range. |
| Databáze: | OpenAIRE |
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