Substance P post-synaptically potentiates glutamate-induced currents in dorsal vagal neurons
| Autor: | Xinhuai Liu, Jean-Jacques Puizillout, Daniel André |
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| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_specialty N-Methylaspartate Glutamic Acid Kainate receptor Substance P AMPA receptor chemistry.chemical_compound Internal medicine medicine Animals Rats Wistar Long-term depression Molecular Biology Neurons General Neuroscience Glutamate receptor Electric Conductivity Drug Synergism Vagus Nerve Glutamic acid Receptors Neurokinin-1 Rats Endocrinology nervous system chemistry Synapses CNQX NMDA receptor Female Neurology (clinical) Neuroscience Developmental Biology |
| Zdroj: | Brain research. 804(1) |
| ISSN: | 0006-8993 |
| Popis: | We examined the post-synaptic actions of glutamate, N-methyl-D-aspartate (NMDA) and substance P on dorsal vagal neurons, using the patch-clamp technique on brainstem slices of young rats. The vagal neurons were identified electrically and histologically. All vagal neurons responded to glutamate and NDMA and about 30% to substance P, with dose-dependent inward currents. The glutamate-induced currents were blocked partially by either CPP (3((R)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid) or CNQX (6-cyano-7-nitro-quinoxaline-2,3-dione), indicating that these currents resulted from the activation of at least two types of glutamate receptors: NMDA receptors and AMPA/kainate receptors. The NK1 receptor-selective antagonist, RP67580, blocked substance P-induced currents, suggesting that NK1 receptors do coexist with NMDA receptors and AMPA/Kainate receptors. Substance P potentiated the effects of glutamate. This potentiation lasted 10-20 min and was blocked by CPP and by RP67580, but not by CNQX, demonstrating that the increase in glutamate-induced currents resulted from the interaction between NK1 receptors and NMDA channels. These results provided the first evidence that the receptors for substance P and glutamate coexist on dorsal vagal neurons and interact with each other to modulate visceral efferent functions. |
| Databáze: | OpenAIRE |
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