Radioimmunoassay of main urinary metabolite of prostaglandin F2alpha
| Autor: | Shiro Ohki, Fumio Hirata, Nobuhiro Nakazawa, Toshio Hanyu, Katsuhiro Imaki |
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| Rok vydání: | 1975 |
| Předmět: |
Prostaglandins F
endocrine system medicine.medical_specialty Time Factors Metabolite Urinary system Indomethacin Radioimmunoassay Prostaglandin Cross Reactions Biochemistry chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Cyclooxygenase Inhibitors Antiserum Aspirin Immune Sera Flufenamic Acid Rats Prostaglandin F2alpha Flufenamic acid chemistry Depression Chemical lipids (amino acids peptides and proteins) Rabbits medicine.drug |
| Zdroj: | Prostaglandins. 10(4) |
| ISSN: | 0090-6980 |
| Popis: | Radioimmunoassay of 5alpha, 7alpha-dihydroxy-11-keto-tetranorprosta-1,16-dioic acid, main urinary metabolite of prostaglandin F2alpha (PGF2alpha), was performed using an antiserum produced in the rabbit. The antibody in 100 mu1 of 1,600-fold diluted antiserum binds with 60 picograms of metabolite. The main urinary metabolite level fell when flufenamic acid, a prostaglandin synthetase inhibitor, was given to rats. In contrast, it was significantly elevated when PGF2alpha was administered. |
| Databáze: | OpenAIRE |
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