Salivary analysis for determination of dextromethorphan metabolic phenotype
| Autor: | Linda W. Pickle, Patricia S. Meyer, Zone Yuan Hou, Raymond L. Woosley |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male medicine.medical_specialty Saliva animal structures Urinary system Debrisoquin Administration Oral Urine Pharmacology Dextromethorphan Dextromethorphan Hydrobromide Internal medicine Dextrorphan medicine Humans Pharmacology (medical) Chromatography High Pressure Liquid Chemistry Racial Groups Middle Aged Endocrinology Phenotype Female Pharmacogenetics medicine.drug |
| Zdroj: | Clinical pharmacology and therapeutics. 49(4) |
| ISSN: | 0009-9236 |
| Popis: | Debrisoquin oxidative phenotype is a determinant of pharmacologie response for many drugs. Poor and extensive metabolizers can be identified by the dextromethorphan metabolic ratio (dextromethorphan/ dextrorphan). We developed and tested a method to determine debrisoquin phenotype on the basis of the metabolic ratio in saliva. Each of 62 normal volunteers was given a 50 mg capsule of dextromethorphan hydrobromide and collected urine (0 to 8 hours) and saliva (at 3 hours). Dextromethorphan and dextrorphan in saliva and urine were assayed by HPLC. The distributions of paired urinary and 3-hour salivary metabolic ratios of samples from 61 subjects were compared. The urinary and salivary metabolic ratios were distributed trimodally and bimodally, respectively. The Spearman rank correlation coefficient for logarithm of urinary metabolic ratio vs that of salivary metabolic ratio was 0.704. All the poor metabolizers identified by urinary metabolic ratio were also identified by the metabolic ratio in saliva at 3 hours (100% concordance). This study demonstrates that salivary analysis for determination of dextromethorphan metabolic phenotype is feasible. Clinical Pharmacology and Therapeutics (1991) 49, 410–419; doi:10.1038/clpt.1991.48 |
| Databáze: | OpenAIRE |
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